Novel coumarin-amide derivatives and its preparation, said drug composition and its use

ABSTRACT

The present invention relates to novel coumarin derivatives, their carboxamides, pharmaceutical compositions containing them and their uses as drugs for kidney protection, treating drugs of hypertension, cardio-cerebrovascular diseases, non-insulin dependent diabetes, tumor, pre-cancerous lesion, and edemas.

TECHNICAL FIELD

The present invention relates to novel coumarins, their carboxamide derivatives, the preparation method thereof and the pharmaceutical compositions containing them, and their use as medicaments for kidney protection, as well as for the treatment of hypertension, cardio-cerebrovascular diseases, non-insulin dependent diabetes (NIDD), tumor, preneoplastic lesions, and edemas.

BACKGROUND ART

In 1990 the German Federal Institute of Drugs and Medicinnal Products (BfArM) published a monograph on Meliloti Herba, in which the use of melilot is indicated for symptoms and signs in chronic venous insufficiency like pains; adjuvant treatment of thrombophlebitis and lymphostasis. Scheel et al. (Microbiol Toxins 8: 47-66, 1972) reported that coumarins have anti-bacterial, anti-viral and anti-tumor effects. Kovach et al (Arzeim-Forsch/Drug Res 20: 1630-33, 1970) proved that coumarins can increase blood flow and improve myocardial ischemia. Casley-Smith, (Vasomed 6: 232-4, 1994), Gaffney (J Pathol 133: 229-42, 1981), Piller (Br J Exp Pathol 59: 319-26, 1978), and Knight (Clin Sci 77: 69-76, 1989) showed that coumarins have effects of endothelial system protection and lymph-circulation promotion, etc. Nair et al. (Carcinogenesis 12 (1): 65-69, 1991) reported the anticancer activity of coumarins compounds. Ishizuka et al. (U.S. Pat. No. 5,096,924) proved that substituted coumarins have anticancer activities. Marshall et al. (J. Biol. Resp. Mod. 8: 62, 1989) reported that coumarins have immuno-enhancing effects, such as improving the antitumor abilities by remarkably raising monocytes of patients suffering from cancer. Preuss-Ueberschar et al. (Drug Res. 34: 1305-1313, 1984) showed that the coumarins are non-teratogenitic. Takagaki, Hidetsugu et al. (EP 0, 796, 854 A1, 1997) disclosed that 3-, 4-, or 7-hydroxy or alkoxy substituted coumarins' effects in treating heart diseases. Markal et al. (WO 98/25, 608, 1998) disclosed that substituted 4-arylcoumarins can be used to treat viral infections, such as herpes zoster or herpes simplex. Trkovnik et al. (WO 99/21550, 1999) reported that 4-methyl-7-hydroxycoumarin can be used to treat or prevent nephrocirrhosis, pancreatitis, and disorders in bladder or alimentary tracts. Takagaki et al. (Jpn. Kokai Tokkyo Koho JP 07277972, 1995) reported that coumarin derivatives can inhibit rat diabetes induced by streptozotocin. Scott et al. (U.S. Pat. No. 5,723,476, 1998) disclosed that 3-carboxamide-4-hydroxy coumarin compounds are effective to the non-insulin dependent diabetes models. Han, Xingmei et al. (Zhongguo Yaolixue Tongbao, 15(4): 332-5, 1999) reported that 6,7-dimethoxycoumarin is effective to acute renal failure induced by endotoxins. Allen et al. (PCT Int Appl WO 2001 019396 A1 2001) reported that the TGF-β1 antagonists may be used for the treatment or prophylaxis of chronic nephritis.

In our research, a series of coumarin derivatives were synthesized and their biological activities were evaluated. For example, Xiao-long Huang et al reported substituted 3-acetyl- and 3-glyoxal-coumarin derivatives possessing good anti-mutagenic effects (Yaoxue Xuebao 31(6): 431-436, 1996; ibid 31(7): 509-516, 1996). Shi-ping Xu et al discovered that the coumarin retinoids show potent differentiation inducing, anti-mutagenic, and anti-carcinogenic effects (Chinese patent application No. 97116602.1, CN1207392A). Song Xu et al.'s study on 6- or 7-substitutedstyryl-coumarins, 4-styryl-coumarins and 4-, 6- or 7-substitutedphenyliminomethylene-coumarins show anticancer effects (Yaoxue Xuebao 35(2): 103-107, 2000; ibid 36(4): 269-273, 2001; ibid 37(2): 113-116, 2002).

Following that, upon our continued research works on coumarins compounds, a series of novel coumarins and coumarin carboxamides were synthesized. And we have found that these coumarins carboxamide compounds possess potent inhibition effects on transforming growth factor β1 (TGF-β1), which has not been reported before. The TGF-β1 inhibitors may be used for the treatment of chronic renal dysfunctions and diabetic renal dysfunctions. Meanwhile, it can also significantly decrease angiotensin II (AngII). Therefore, the compounds of present invention, may be used in the drugs for the treatment of chronic renal failure, nephritis, hypertension, even cirrhosis of liver and pulmonary fibrosis. For example, Allen et al. (PCT Int Appl WO 2001 019396 A1 2001) reported that the TGF-β antagonists may be used for the treatment or prophylaxis of chronic nephritis.

Renal insufficiencies, particularly chronic renal failure, are the results of kidney injuries with various pathogenesis and progressive deterioration. Among the primary nephropathies, the most common is the chronic glomerulonephritis, and tubolointerstitial nephritic comes the second. Among the secondary nephropathies, diabetic nephropathy holds the first position. At present, diabetic nephropathy holds about 27% of the origin of chronic renal failures, and is still increasing; hypertensive nephropathy comes the second, about 22.7% and the glomerulonephritis comes next about 21.2% and all other pathogenesis occupy 26.6% in the origin of chronic renal insufficiencies. Being a common disease per se, nephropathies, no wonder what pathogenesis, or being immune or non-immune mechanism, unless promptly treated, may be result in chronic renal insufficiency and irreversible chronic renal injuries.

Upon the researches of the field, it shows that transforming growth factor-β1 (TGF-β1) has a close relationship with chronic renal insufficiencies caused by various pathogenesis. Four hours after nephrectomy, TGF-β1 began to increase and the renin-angiotensin system was consequently influenced. Continuously rising of TGF-β1 and over-expression will result in inhibiting the degradation of the extracellular matrix, promoting the matrix integration, and also relates to the proteinuria from renal insufficiency, as well as matrix fibrosis. Therefore, glomerular sclerosis and interstitial fibrosis have direct relationship with TGF-β1, and renin-angiotensin system and TGF-β1 are the two critical factors related to chronic renal insufficiencies. Moreover, as the inhibition of the former has close relationship with the decrease of TGF-β1 producing, this suggests that the increase of TGF-β1 might be an important pathogenesis of kidney injuries to the end-stage renal insufficiencies, and would be a new target for the screening of new anti-renal failure drugs.

Coumarin compounds possess extensive biological activities, however, it haven't been reported in the literatures that these compounds can be used for the treatment of chronic renal failures. The present compounds are a novel type compounds and can remarkably inhibit TGF-β1, which is an important pathogenesis of kidney injuries to the end-stage renal failure. It has been proved that, the compound of present invention show satisfied effects on treating renal insufficiencies.

Renal insufficiencies, especially chronic renal insufficiencies, are chronic diseases that are hard to be cured. With the continuously increase of diabetes and hypertension, the incidence of renal insufficiencies becomes more and more, and has no effective drugs or other methods for the treatment or prophylaxis up to now. Therefore, the object of the present invention is to develop new drug for the treatment of renal insufficiencies.

SUMMARY OF THE INVENTION

An object of the present invention is to overcome one or more deficiencies of the prior arts, and to provide a new coumarin, in particular to provide low toxic carboxamide derivatives thereof.

One another object of the present invention is to provide a preparation method of the coumarin carboxamide derivatives.

One aspect of the present invention relates to pharmaceutical compositions, which comprises a compound of general formula (I) or an isomer thereof as the active ingredient, and pharmaceutically acceptable carriers.

A further object of the present invention is to provide use of the novel coumarin carboxamide derivatives or the compositions thereof for the as TGF-β1 and angiotensin II (AngII) inhibitors.

A still further object of the present invention is to provide use of the novel coumarin carboxamide derivatives or the compositions thereof for the preparation of the medicaments for the treatment of kidney disorders (such as various chronic nephritis, diabetic and hypertensive renal insufficiency), non-insulin dependent diabetes, cardio-cerebrovascular diseases and hypertension.

Specifically, the first aspect of the present invention relates to the compounds of the general formula (I)

Wherein,

R³ is selected from the group consisting of H, carboxyl, alkyloxycarbonyl, 5′-(phenyloxadiazol-2′)-yl, 5′-(pyridyl-4″-oxadizol-2′)-yl,

CONHR₉, wherein R₉ is selected from the group consisting of C₂-C₈ fatty acid, benzoxamido, isonicotinamido, un-substituted or mono- or multi-substituted phenyl wherein the substituent may be hydroxyl, C₁-C₈ alkoxyl, CF₃, carboxyl, alkyloxycarbonyl, OCH₂CO₂H, NO₂, halogen, SO₃H, SO₂NHR₁₁, wherein R₁₁ is selected from the group consisting of hydrogen, amidino, 2″-thiazolyl, 3″-(5″-methylisooxazolyl), 2″-pyrimidinyl, 2″-(4″,6″-dimethylpyrimidinyl), 4″-(5″,6″-dimethoxypyrimidinyl);

R₄ is selected from the group consisting of hydrogen, CONHR₁₀, wherein R₁₀ is selected from the group consisting of C₂-C₈ fatty acid, benzoxamido, isonicotiniamido, un-substituted, mono- or multi-substituted phenyl wherein the substituent may be hydroxyl, C₁-C₈ alkoxyl, CF₃, carboxyl, alkoxycarbonyl, OCH₂CO₂H, NO₂, halogen, SO₃H, SO₂NHR₁₂, wherein R₁₂ is selected from the group consisting of H, amidino, 2″-thiazolyl, 3″-(5″-methylisooxazolyl), 2″-pyrimidinyl, 2″-(4″,6″-dimethyl-pyrimidinyl), 4″-(5″,6″-dimethoxy pyrimidinyl);

R₅ is selected from the group consisting of H, C₁-C₄ alkyl;

R₆ is selected from the group consisting of H, C₁-C₁₂ alkyl, halogen, NO₂, CONHR₁₃, wherein R₁₃ is substituted phenyl;

R₇ is selected from the group consisting of H, hydroxyl, C₁-C₄ alkyl or alkoxyl, carboxylalkylenoxyl, OCH₂CONHR₁₄, wherein R₁₄ is selected from the group consisting of un-substituted, mono- or multi-substituted phenyl wherein the substituent may be hydroxyl, OCH₃, CF₃, CO₂H, CO₂C₂H₅, NO₂;

R₈ is selected from the group consisting of H, C₁-C₄ alkyl or alkoxyl, NO₂;

In order to achieve the object of the present invention, preferable compounds include, but are not limited to the following compounds: R₃ is selected from the group consisting of H, COOH, CO₂C₂H₅, 5′-(phenyloxadiazol-2′)-yl, 5′-(pyridyl-4″-oxadizol-2′)-yl,

CONHR₉, wherein R₉ is n-butyric acid, o-, m-, p-phenol, o-, m-, p-carboxyl-phenyl, o-, m-, p-alkyloxycarbophenyl, methoxylphenyl, 3′-hydroxy-4′-carboxyphenyl, 3′-salicylyl, 4′-salicylyl, m-CF₃-phenyl, 3′-CF₃-4′-NO₂-phenyl, 2′-CO₂H-4′-I-phenyl, isonicotinamido, benzoxamido, 3′-carboxy-methylenoxyphenyl, 4′-amidosulfonylphenyl, 4′-guanidinosulfonylphenyl, 4′-(2″-thiazolamidosulfonyl)phenyl, 4′-(5″-methylisooxazolyl-3″-amidosulfonyl)phenyl, 4′-(pyrimidinyl-2″-amidosulfonyl)phenyl, 4′-(4″,6″-dimethylpyrimidinyl-2″-amidosulfonyl)phenyl, 4′-(5″,6″-dimethoxypyrimidinyl-4″-amidosulfonyl)phenyl;

R₄ is selected from the group consisting of H, CONHR₁₀, wherein R₁₀ is selected from the group consisting of H, 4′-CO₂H-phenyl, 4′-CO₂C₂H₅phenyl, 3′-CF₃-phenyl;

R₅ is selected from the group consisting of H, CH₃;

R₆ is selected from the group consisting of H, C₂H₅, n-C₆H₁₃, NO₂, NH₂, Cl, Br, CONHR₁₃, wherein R₁₃ is selected from the group consisting of carboxyl- and alkoxycarbonyl-substituted phenyl;

R₇ is selected from the group consisting of H, OH, CH₃, OCH₃, OCH₂CONHR₁₄, wherein R₁₄ is selected from the group consisting of phenyl, o-, m- and p-hydroxyphenol, o-, m- and p-carboxylphenyl, m- and p-ethoxycarbonylphenyl, m-CF₃-phenyl, m-CF₃-p-NO₂-phenyl, p-CH₃O-phenyl, 4-salicylyl, 3-salicylyl;

R₈ is selected from the group consisting of H, CH₃, OCH₃, NO₂;

In order to complete the object of the present invention, preferable compounds include, but are not limited to the compounds represented by following general formula (Ia):

wherein R₄, R₅, R₆, R₇, R₈ are defined same as general formula

In order to complete the object of the present invention, preferable compounds include, but are not limited to the compounds represented by the following general formula (Ib):

wherein R₄, R₅, R₆, R₇, R₈, are same as defined in general formula (I),

R′₂ is selected from the group consisting of H, OH, CO₂H, etc;

R′₃ is selected from the group consisting of H, OH, CO₂H, CF₃, OCH₂CO₂H, etc;

R′₄ is selected from the group consisting of H, OH, CO₂H, CO₂Et, Iodo, NO₂, OCH₃, SO₃H, SO₂NH₂, SO₂NH(C═NH)NH₂,

etc;

R′₅ and R′₆ each is H;

In order to complete the object of the present invention, preferable compounds include, but are not limited to the compounds of the following tables 1 and 2:

Wherein R₄-R₈ groups are H except specified, R′₂-R′₆ groups are H except specified TABLE 1 No. R₄—R₈

MP ° C. 1 7-OCH₃ 4′-COOH >300 2 7-OCH₃ 3′-COOH >300 3 7-OCH₃ 2′-COOH >300 4 7-OCH₃ 2′-OH >300 5 7-OCH₃ 3′-OH 265 6 7-OCH₃ 4′-OH >300 7 7-OCH₃ 3′-OH, 4′-COOH 279d 8 7-OCH₃ 3′-COOH, 4′-OH 230d 9 7-OCH₃ 2′-COOH, 4′-I >300 10 7-OCH₃ 4′-COOEt 247 11 7-OCH₃ 3′-CF₃ 218 12 7-OCH₃ 3′-CF₃ 4′-NO₂ >300 13 7-OCH₃ 4′-SO₂NH₂ >300 14 7-OCH₃ 4′-SO₂NH(C═NH)NH₂ >300 15 7-OCH₃

>300 16 7-OCH₃

>300 17 7-OCH₃

298 18 7-OCH₃

300 19 7-OCH₃

282d 20 7-OCH₃ 4′-OCH₃ 233 21 7-OCH₃ 4′-SO₃H 284 22 6-Et  7-OCH₃ 4′-COOH >300 23 6-Et  7-OCH₃ 3′-COOH 298 24 6-Et  7-OCH₃ 2′-COOH 294 25 6-Et  7-OCH₃ 4′-OH 304 26 6-Et  7-OCH₃ 3′-OH, 4′-COOH 266 27 6-Et  7-OCH₃ 3′-COOH, 4′-OH 298 28 6-Et  7-OCH₃ 4′-COOEt 233 29 6-Et  7-OCH₃ 3′-CF₃ 224 30 6-Et  7-OCH₃ 3′-CF₃ 4′-NO₂ 234 31 6-Et  7-OCH₃ 4′-SO₂NH₂ >300 32 6-Et  7-OCH₃ 4′-SO₂NH(C═NH)NH₂ 299 33 6-Et  7-OCH₃

>300 34 6-Et  7-OCH₃

>300 35 6-Et  7-OCH₃

278 36 6-Et  7-OCH₃

260d 37 6-Et  7-OCH₃

>300 38 6-Et  7-OCH₃ 4′-OCH₃ 202 39 6-Et  7-OCH₃ 4′-SO₃H >300 40 7-OCH₃  8-CH₃ 4′-COOH >300 41 7-OCH₃  8-CH₃ 3′-COOH >300 42 7-OCH₃  8-CH₃ 2′-COOH 264 43 7-OCH₃  8-CH₃ 3′-OH, 4′-COOH 279 44 7-OCH₃  8-CH₃ 3′-COOH, 4′-OH 290 45 7-OCH₃  8-CH₃ 2′-COOH, 4′-I 284 46 7-OCH₃  8-CH₃ 4′-COOEt 270 47 7-OCH₃  8-CH₃ 3′-CF₃ 258 48 7-OCH₃  8-CH₃ 3′-CF₃, 4′-NO₂ 252 49 7-OCH₃  8-CH₃ 4′-SO₂NH₂ 300 50 7-OCH₃  8-CH₃ 4′-SO₂NH(C═NH)NH₂ >300 51 7-OCH₃  8-CH₃

>300 52 7-OCH₃  8-CH₃

277 53 7-OCH₃  8-CH₃

220d 54 7-OCH₃  8-CH₃

286 55 7-OCH₃  8-CH₃

286 56 7-OCH₃  8-CH₃ 4′-OCH₃ 258 57 7-OCH₃  8-CH₃ 4′-SO₃H 286 58 7-OCH₃  8-OCH₃ 4′-COOH 315 59 7-OCH₃  8-OCH₃ 3′-OH, 4′-COOH 264 60 7-OCH₃  8-OCH₃ 3′-COOH, 4′-OH 264 61 7-OCH₃  8-OCH₃ 4′-COOEt 236 62 7-OCH₃  8-OCH₃ 3′-CF₃ 243 63 7-OCH₃  8-OCH₃ 3′-CF₃, 4′-NO₂ 283 64 7-OCH₃  8-OCH₃ 3′-OCH₂COOH 188 65 7-OCH₃  8-OCH₃ 4′-SO₂NH₂ 280 66 7-OCH₃  8-OCH₃ 4′-SO₂NH(C═NH)NH₂ 252 67 5-CH₃  7-OCH₃ 4′-COOH 299 68 5-CH₃  7-OCH₃ 3′-COOH >300 69 5-CH₃  7-OCH₃ 2′-COOH >300 70 5-CH₃  7-OCH₃ 2′-OH 246 71 5-CH₃  7-OCH₃ 3′-OH 292 72 5-CH₃  7-OCH₃ 4′-OH 255 73 5-CH₃  7-OCH₃ 3′-OH, 4′-COOH 284 74 5-CH₃  7-OCH₃ 3′-COOH  4′-OH >300 75 5-CH₃  7-OCH₃ 4′-COOEt 265 76 5-CH₃  7-OCH₃ 3′-CF₃ 221 77 5-CH₃  7-OCH₃ 3′-CF₃, 4′-NO₂ >300 78 5-CH₃  7-OCH₃ 4′-SO₂NH₂ 283 79 5-CH₃  7-OCH₃ 4′-SO₂NH(C═NH)NH₂ 293 80 5-CH₃  7-OCH₃

288 81 5-CH₃  7-OCH₃

>300 82 5-CH₃  7-OCH₃

274d 83 5-CH₃  7-OCH₃

268 84 5-CH₃  7-OCH₃

271 85 5-CH₃  7-OCH₃ 4′-OCH₃ 210 86 6-Cl  7-OCH₃ 4′-COOH >300 87 6-Cl  7-OCH₃ 3′-OH, 4′-COOH 253 88 6-Cl  7-OCH₃ 3′-COOH, 4′-OH >300 89 6-Cl  7-OCH₃ 4′-COOEt 294 90 6-Cl  7-OCH₃ 3′-CF₃ 282 91 6-Cl  7-OCH₃ 4′-SO₂NH₂ >300 92 6-Cl  7-OCH₃ 4′-SO₂NH(C═NH)NH₂ 302 93 6-Cl  7-OCH₃

317 94 6-Br  7-OCH₃ 4′-COOH >300 95 6-Br  7-OCH₃ 2′-COOH 288 96 6-Br  7-OCH₃ 3′-OH, 4′-COOH 298 97 6-Br  7-OCH₃ 2′-COOH, 4′-I >300 98 6-Br  7-OCH₃ 4′-COOEt 298 99 6-Br  7-OCH₃ 3′-CF₃ 284 100 6-Br  7-OCH₃ 4′-SO₂NH₂ 298 101 6-Br  7-OCH₃ 4′-OCH₃ 262 102 6-nHex  7-OCH₃ 4′-COOH 248 103 6-nHex  7-OCH₃ 2′-COOH 272 104 6-nHex  7-OCH₃ 3′-OH, 4′-COOH 268 105 6-nHex  7-OCH₃ 2′-COOH, 4′-I 249 106 6-nHex  7-OCH₃ 4′-COOEt 160 107 6-nHex  7-OCH₃ 3′-CF₃ 201 108 6-nHex  7-OCH₃ 4′-SO₂NH₂ 242 109 6-nHex  7-OCH₃ 4′-OCH₃ 170 110 6-NO₂  7-OCH₃  8-OCH₃ 4′-COOH >300 111 6-NO₂  7-OCH₃  8-OCH₃ 3′-COOH 232 112 6-NO₂  7-OCH₃  8-OCH₃ 4′-OCH₃ 256 113 6-NO₂  7-OCH₃  8-OCH₃ 3′-OH 160 114 6-NO₂  7-OCH₃  8-OCH₃ 2′-OH 217 115 6-NO₂  7-OCH₃  8-OCH₃ 4′-COOEt 193 116 6-NO₂  7-OCH₃  8-OCH₃ 3′-OH, 4′-COOH >300 117 6-NO₂  7-OCH₃  8-OCH₃ 3′-COOH, 4′-OH 266d 118 6-NO₂  7-OCH₃  8-OCH₃ 3′-CF₃ 218 119 6-NO₂  7-OCH₃  8-OCH₃ 3′-CF₃, 4′-NO₂ 217 120 6-NO₂  7-OCH₃  8-OCH₃ 4′-SO₂NH₂ 290d 121 6-NO₂  7-OCH₃  8-OCH₃ 4′-SO₂NH(C═NH)NH₂ 284 122 6-NO₂  7-OCH₃  8-OCH₃

190d 123 6-NO₂  7-OCH₃  8-OCH₃

220d 124 6-NO₂  7-OCH₃  8-OCH₃

200d 125 6-C₂H₅, 7-OH  8-NO₂ 4′-COOH 234 126 6-C₂H₅, 7-OH  8-NO₂ 4′-OCH₃ 218d 127 6-C₂H₅, 7-OH  8-NO₂ 3′-OH >300 128 6-C₂H₅, 7-OH  8-NO₂ 2′-OH 248d 129 6-C₂H₅, 7-OH  8-NO₂ 4′-COOEt 160 130 6-C₂H₅, 7-OH  8-NO₂ 3′-OH, 4′-COOH >300 131 6-C₂H₅, 7-OH  8-NO₂ 3′-COOH, 4′-OH >300 132 6-C₂H₅, 7-OH  8-NO₂ 3′-CF₃ 169 133 6-C₂H₅, 7-OH  8-NO₂ 4′-SO₂NH₂ 206d 134 6-C₂H₅, 7-OH  8-NO₂ 4′-SO₂NH(C═NH)NH₂ 181 135 6-C₂H₅, 7-OH  8-NO₂

>300 136 6-C₂H₅  7-OCH₃  8-NO₂ 4′-COOH 273 137 6-C₂H₅  7-OCH₃  8-NO₂ 4′-OH 252 138 6-C₂H₅  7-OCH₃  8-NO₂ 4′-OCH₃ 169 139 6-C₂H₅  7-OCH₃  8-NO₂ 4′-COOEt 186 140 6-C₂H₅  7-OCH₃  8-NO₂ 4′-SO₂NH(C═NH)NH₂ 206d 141 6-NO₂, 7-OH, 8-CH₃ 4′-COOH >300 142 6-NO₂, 7-OH, 8-CH₃ 2′-COOH 227 143 6-NO₂, 7-OH, 8-CH₃ 4′-OH >300 144 6-NO₂, 7-OH, 8-CH₃ 3′-OH >300 145 6-NO₂, 7-OH, 8-CH₃ 2′-OH >300 146 6-NO₂, 7-OH, 8-CH₃ 4′-OCH₃ 254 147 6-NO₂, 7-OH, 8-CH₃ 4′-COOEt >300 148 6-NO₂, 7-OH, 8-CH₃ 3′-OH, 4′-COOH 271 149 6-NO₂, 7-OH, 8-CH₃ 3′-COOH, 4′-OH >300 150 6-NO₂, 7-OH, 8-CH₃ 3′-CF₃ 231 151 6-NO₂, 7-OH, 8-CH₃ 3′-CF₃, 4′-NO₂ 226 152 6-NO₂, 7-OH, 8-CH₃ 4′-SO₂NH₂ >300 153 6-NO₂, 7-OH, 8-CH₃ 4′-SO₂NH(C═NH)CH₂ >300 154 6-NO₂, 7-OH, 8-CH₃

>300 155 6-NO₂, 7-OH, 8-CH₃

254 156 6-NO₂, 7-OH, 8-CH₃

>300 157 6-NO₂, 7-OH, 8-CH₃ 2′-COOH, 4′-I 262 158 6-NO₂, 7-OCH₃, 8-CH₃ 4′-COOH 301 159 6-NO₂, 7-OCH₃, 8-CH₃ 3′-COOH 280 160 6-NO₂ 7-OCH₃, 8-CH₃ 2′-COOH 282 161 6-NO₂, 7-OCH₃, 8-CH₃ 4′-OH >300 162 6-NO₂, 7-OCH₃, 8-CH₃ 3′-OH 231 163 6-NO₂, 7-OCH₃, 8-CH₃ 2′-OH 285 164 6-NO₂, 7-OCH₃, 8-CH₃ 4′-OCH₃ 209 165 6-NO₂, 7-OCH₃, 8-CH₃ 4′-COOEt 230 166 6-NO₂, 7-OCH₃, 8-CH₃ 3′-OH, 4′-COOH 249 167 6-NO₂, 7-OCH₃, 8-CH₃ 3′-CF₃ 182 168 6-NO₂, 7-OCH₃, 8-CH₃ 3′-CF₃, 4′-NO₂ 236 169 6-NO₂, 7-OCH₃, 8-CH₃ 4′-SO₂NH(C═NH)NH₂ >300 170 6-NO₂, 7-OCH₃, 8-CH₃ 4′-SO₂NH₂ 301 171 6-NO₂, 7-OCH₃, 8-CH₃

276 172 6-NO₂, 7-OCH₃, 8-CH₃

270 173 6-NO₂, 7-OCH₃, 8-CH₃

299 174 6-NO₂, 7-OH, 8-NO₂ 4′-COOH >300 175 6-NO₂, 7-OH, 8-NO₂ 4′-OH 260 176 6-NO₂, 7-OH, 8-NO₂ 3′-OH >300 177 6-NO₂, 7-OH, 8-NO₂ 2′-OH >300 178 6-NO₂, 7-OH, 8-NO₂ 4′-OCH₃ >300 179 6-NO₂, 7-OH, 8-NO₂ 4′-COOEt 281 180 6-NO₂, 7-OH, 8-NO₂ 3′-CF₃ 197 181 6-NO₂, 7-OH, 8-NO₂ 4′-SO₂NH₂ >300 182 6-NO₂, 7-OH, 8-NO₂ 4′-SO₂NH(C═NH)NH₂ 216 183 6-NO₂, 7-OH, 8-NO₂

>300 184 6-NO₂, 7-OH, 8-NO₂

170 185 6-NO₂, 7-OH, 8-NO₂

>300 186 6-NO₂, 7-OH, 8-NO₂ 2′-COOH 285 187 6-NO₂, 7-OCH₃  8-NO₂ 4′-OH 257 188 6-NO₂, 7-OCH₃  8-NO₂ 4′-COOEt 236 189 6-NO₂, 7-OCH₃  8-NO₂ 4′-OCH₃ 205 190 6-Cl  7-OH  8-NO₂ 4′-OCH₃ 285 191 6-Cl  7-OH  8-NO₂ 4′-SO₂NH(C═NH)NH₂ 300d 192 6-Cl  7-OH  8-NO₂ 3′-OH  4′-COOH >300 193 5-CH₃, 6-, 8-(NO₂)₂  7-OH 4′-COOH >300 194 5-CH₃6-, 8-(NO₂)₂, 7-OH 3′-COOH 246 195 5-CH₃6-, 8-(NO₂)₂  7-OH 2′-COOH 214 196 6-CH₃6-, 8-(NO₂)₂  7-OH 4′-OCH₃ 242 197 5-CH₃6-, 8-(NO₂)₂  7-OH 4′-COOEt 244 198 5-CH₃6-, 8-(NO₂)₂  7-OH 4′-SO₂NH₂ 256 199 5-CH₃, 6-, 8-(NO₂)₂  7-OH 4′-SO₂NH(C═NH)NH₂ >300 200 5-CH₃6-, 8-(NO₂)₂  7-OH

>300 201 5-CH₃6-, 8-(NO₂)₂  7-OH

220 202 5-CH₃6-, 8-(NO₂)₂  7-OH

276 No. R₄—R₈ R₃ MP ° C. 203 7-OCH₃ CONH(CH)₃CO₂H 193 204 7-OCH₃

293 205 7-OCH₃

248 206 7-OCH₃

238 207 6-C₂H₅, 7-OCH₃ CONH(CH)₃CO₂H 226 208 6-C₂H₅, 7-OCH₃

293 209 6-C₂H₅, 7-OCH₃

196 210 5-CH₃, 7-OCH₃

248 211 5-CH₃, 7-OCH₃

176 212 5-CH₃, 7-OCH₃

240 213 7-OCH₃, 5-CH₃

254 214 7-OCH₃, 8-CH₃

254 215 7-OCH₃  8-CH₃

278 216 7-OCH₃  8-CH₃

270 217 6-Br  7-OCH₃

248 218 6-Br  7-OCH₃

>300 219 6-Br  7-OCH₃

295 220 6-n-C₆H₁₃  7-OCH₃

198 221 6-n-C₆H₁₃  7-OCH₃

196 222 6-n-C₆H₁₃  7-OCH₃

139 223 6-NO₂, 7-OH, 8-CH₃

>300 224 6-NO₂, 7-OCH₃, 8-CH₃

220 225 6-NO₂  7,8-(OCH₃)₂

199 226 6-NO₂  7,8-(OCH₃)₂

>300

TABLE 2 No. R₃ R₄ R₅ R₆ R₇ R₈ MP ° C. 227 CO₂C₂H₅ H H NO₂ OCH₃ OCH₃ 191 228 CO₂H H H NO₂ OCH₃ OCH₃ 188 229 CO₂C₂H₅ H H NO₂ OH CH₃ 210 230 CO₂H H H NO₂ OH CH₃ 251 231 CO₂C₂H₅ H H NH₂ OH CH₃ 203 232 CO₂H H H NO₂ OCH₃ CH₃ 178 233 CO₂C₂H₅ H H C₂H₅ OH NO₂ 143 234 CO₂H H H C₂H₅ OH NO₂ 178 235 CO₂C₂H₅ H H C₂H₅ OCH₃ NO₂ 140 236 CO₂H H H C₂H₅ OCH₃ NO₂ 176 237 CO₂C₂H₅ H H NO₂ OH NO₂ 176 238 CO₂H H H NO₂ OH NO₂ 296 239 CO₂C₂H₅ H H NO₂ OCH₃ NO₂ 152 240 CO₂H H H NO₂ OCH₃ NO₂ 246 241 CO₂C₂H₅ H H Cl OH NO₂ 195 242 CO₂H H H Cl OH NO₂ >300 243 CO₂H H CH₃ NO₂ OH NO₂ 211 244 CO₂C₂H₅ H CH₃ NO₂ OH NO₂ 104 245 H

H H CH₃ H 223 246 H

H H CH₃ H >300 247 H

H H CH₃ H 197 248 H CH₃ H H

H 230 249 H CH₃ H H

H 220 250 H CH₃ H H

H 240 251 H CH₃ H H

H 196 252 H CH₃ H H

H 304 253 H CH₃ H H

H >300 254 H CH₃ H H

H 296 255 H CH₃ H H

H 207 256 H CH₃ H H

H 157 257 H CH₃ H H

H 243 258 H CH₃ H H

H 301 259 H CH₃ H H

H 180 260 H CH₃ H H

H 215 261 H CH₃ H H

H 277 262 H CH₃ H H

CH₃ 216 263 H CH₃ H H

CH₃ 205 264 H CH₃ H H

CH₃ 260 265 R₃ = R₄ = R₅ = R₇ = R₈ = H

214 266 R₃ = R₄ = R₅ = R₇ = R₈ = H

300

In this description, the term “halogen” indicates Fluoro, Chloro, Bromo and Iodo. The terms “lower alkane”, “lower alkyl” mean linear or branched alkanes and alkyls having 1-6 carbon atoms.

According to the present invention, the compounds may have isomers, generally the said “compounds of the present invention” includes isomers thereof.

The compounds of the present invention may contain cis-/trans-isomers of a double bond, asymmetric center with S- and R-configurations, and include all the potential stereoscopic isomers and mixtures of two or more isomers. In case that cis-/trans-isomers exist, the present invention also relates to the cis- and trans-isomer and their mixtures, and a pure isomer may be separated according to the conventional methods or synthesized from stereo-selective reagents if necessary.

According to the embodiments of present invention, said compounds may also include the pharmaceutically acceptable salts and hydrate(s), esters, or pro-drugs thereof.

According to the present invention, it is also related to the preparation methods of the compounds of the present invention, which are prepared via nitrating or bi-nitrating various substituted 3-esteryl or 3-carboxy-coumarins, thus to obtain part of the target compounds of present invention and meanwhile the intermediates for the target compounds; reacting the intermediated acids, 3-carboxy-substituted various substituted coumarins, 4-carboxy-substituted various substituted coumarins, 6-carboxy-substituted various substituted coumarins and 7-carboxy-substituted various substituted coumarins with corresponding substituted amines to achieve the target compounds.

The amidating reaction is carried out with corresponding reactants and catalysts, and in the suitable solvents. Said reactants include phosphorus trichloride, phosphorus oxychloride, phosphorus pentachloride, thionyl chloride, 1,3-dicyclohexylcarbodiimide, dipyridylcarbonate (2-DPC), 1,3-diisopropylcarbodiimide (DIPC), and 1-(3-dimethylamino-propyl)-3-ethylcarbodiimide (EDCI), etc. Preferable reactants are phosphorus pentachloride, phosphorus trichloride and thionyl chloride, more preferable reactants are phosphorus pentachloride and thionyl chloride. Catalytic agents for the preparation of the compounds of present invention include tertiary amines, pyridine, 4-dimethylaminopyridine and 4-pyrrolidylpyridine, preferably tertiary amines and pyridine, more preferably pyridine. The reaction is carried out in a suitable solvent or the above condensation agent, such as anhydrous aprotic solvent, dimethylsulfoxide (DMSO), toluene, dichloromethane, 1,2-dichloroethane, ethylene glycol dimethyl ether, tetrahydrofuran and N,N-dimethylformamide (DMF), preferably toluene, DMSO and DMF, more preferably toluene and DMF.

The reaction temperature is 10˜110° C., preferably 20˜90° C., more preferably 30˜80° C., particularly preferably 50˜70° C.

The synthetic routes are specifically explained in the following routes IIa, IIb, IIc, IId, IIe and IIf.

Thus, the present invention also relates to a pharmaceutical composition containing a compound of present invention as active ingredient and conventionally pharmaceutical excipients or auxiliaries. Generally the composition may comprise the compound of present invention from 0.1 to 95% by weight.

The composition of present invention may be prepared by the common methods according to the art. For this purpose, the compounds of the present invention may be combined with one or more solid, semi-solid or liquid excipients and/or auxiliaries, to prepare a suitable administration form or dosage forms for human or animal use.

The compounds of the present invention or compositions containing them may be administered in the unit dosage forms and the administration routes, which can be oral or parenteral, such as oral, intramusculary, subcutaneously, nasal, oral mucosa, transdermal, intraperitoneal or rectal, etc.

The compounds of the present invention or compositions containing them may be administered via injection, including intravenous, intramuscular, subcutaneous, intradermal, and acupoint etc.

The administration forms may be liquid or solid forms. For example the liquid forms may be solutions, colloids, fine particles, emulsion, suspensions and the like. Other forms may be such as tablets, capsules, sprays, dripping pills, aerosols, pills, powders, solutions, suspensions, emulsions, granules, suppositorys, freeze-dried powder for injections and the like.

The present compounds can be prepared as normal preparations, or sustained-release, controlled-release or targeted preparations and various fine particle delivery systems.

In order to prepare unit administration forms to tablets, carriers well known in the art can be used. Carriers such as, diluents and absorbents, such as starch, dextrin, calcium sulfate, lactose, mannitol, sucrose, sodium chloride, glucose, urea, calcium carbonate, kaolin, microcrystallinecellulose, aluminum silicate and the like; moist and binding agents for example water, glycerol, polyethyleneglycol, ethanol, propanol, starch paste, dextrin, syrups, honey, glucose solution, Arabia gum, gelatin, sodium carboxymethylcelluloses, lacta, methylcellulose, potassium phosphate, polyvinylpyrrolidone and the like; disintegrants, for example dry starch, alginates, agar powders, laminarin, sodium hydrogencarbonate-citric acid, calcium carbonate, polyoxyethylene-sorbitol fatty acid esters, sodium lauryl sulfonate, methylcellulose, ethylcellulose and the like; disintegrant inhibitors, such as sucrose, glycerol tristearate, cocoa butter, hydrogenated oil and the like; absorb-promotors, for example quaternary ammonium salts, sodium lauryl sulfate and the like; lubricants, for example talc, silica, corn starch, stearates, boric acid, liquid paraffin, polyethyleneglycol and the like. The tablets may be further coated, for example sugarcoating, film coating, enteric coating, or two or multi-layered tablets.

To prepare pills from the administration units, carriers well known in the art can be used. Carriers such as, diluents and absorbents, such as glucose, lactose, starch, cocoa butter, hydrogenated vegetable oils, polyvinylpyrrolidone, Gelucire, kaolin, talc and the like; binding agents such as arabia gum, tragacanth, gelatin, ethanol, honey, liquid-sugars, rice pastes or flour pastes and the like; disintegrants such as agar powder, dry starch, alginates, sodium lauryl sulfonate, methylcellulose, ethylcellulose and etc.

To prepare capsules from the administration units, the compounds of present invention may be mixed with the above carrier(s), and the so obtained mixtures are packaged into hard or soft capsules. Alternatively, the present compounds may also be prepared into microcapsules, and can be used as suspension in a hydrous media, or packaged into hard capsules or injections.

To prepare the injection dosage forms, the compounds of present invention may be formulated into solutions, suspensions, emulsions, freeze dried powders for injections. Such formulations may be hydrous or anhydrous, which may contain one or two or more pharmaceutically acceptable carrier(s), diluents, preservatives, surfactants or dispersing agents. For example, diluents are selected from water, ethanol, polyethylene glycol, 1,3-propylene glycol, ethoxyisostearyl alcohol, polyoxyisostearyl alcohol, polyoxyethylene-sorbitol fatty acid esters and the like. In addition, to prepare the isotonic injections, sodium chloride, glucose or glycerol can be added to the injection solution, further, solubilizing agents, buffering agents, pH-modulators and the like can also be added.

Also, if desired, coloring agents, preservatives, perfumes, correctants, sweetening agents and the like, can also be added to the pharmaceutical preparation.

For achieving administering purpose and enhancing treating effect, the compounds or pharmaceutical compositions of the present invention may be administered by any known methods. The administering dosage of the present invention may extensively be varied by depending on a number of factors, for example the seriousness of the diseases to be treated or prevented, sex, age, body weights, disposition and individual differences of the patients or animals, administering routes or number of times, treating purposes, etc. In general, the effective dosages of the pharmaceutically active ingredients are known to those skilled in the art, the real administering dose can be suitably adjusted based on the exact dosage contained in the formulations to achieve the treatment or prophylaxis purposes.

The daily suitable ranges of dosages of the compounds of present invention are within 0.001-150 mg/kg body weight, preferably 0.1-100 mg/kg, more preferably 1-60 mg/kg, most preferably 2-30 mg/kg. These doses can be administered in one single dose or divided into several doses, for example twice, three or four times per day, which depends on the experiences of doctors and the other different therapeutic means.

The total dose of each treatment may be administered in one portion or divided into multi portions, depending on the total dose. The compound or the pharmaceutical compositions of present invention may be adopted alone or in combination with other drugs, in the latter case, the dose may also be adjusted.

The activities and effects of the compounds and/or compositions of the present invention may be determined via in vitro and in vivo tests, such as TGF-β1 antagonism, treating of the renal insufficiencies and the like, which are all known in the filed. In recent years, researches have confirmed that TGF-β1 is one of most critical factors resulting in the progressive renal failure with glomerulosclerosis and interstitial fibrosis.

Pharmacological tests show that the compounds of the present invention possess effects of blocking the binding of TGF-β1 with the receptors and inhibiting the production of TGF-β1. Of all the 33 subject compounds in 10 μg/ml doses, 11 compounds possess activities of exceeding 50%, 8 compounds possess activities of exceeding 60%, 7 compounds possess activities of exceeding 70%, 5 compounds possess activities of exceeding 80%, and 4 compounds possess activities of exceeding 90%.

In the cell growth inhibition model of mink pulmonary epithelial cells induced by TGF-β1, of all the 5 subject compounds, 3 compounds possess activities of exceeding 60%, 2 compounds possess activities of exceeding 70%, and 1 compound possesses activity of exceeding 90%. Thus, the present compounds can be used for the treatment or prophylaxis of chronic renal disorders, including: a) primary nephropathies, commonly such as, the chronic glomerulonephritis, interstitial nephritis, chronic pyelonephritis and the like; b) secondary nephropathies, commonly such as, chronic diabetic nephropathy, hypertensive nephropathy, lupus nephropathy and the like; c) congenital and obstructive diseases such as polycystic kidney, posterior urethral valve disorders, neurogenic bladder hyperplasia, prostatic hyperplasia, urinary lithiasis, etc.

Additional researches show that the compounds of the present invention can remarkably inhibit the effect of Ang II (P<0.01). As mentioned above, TGF-β1 and the renin-angiotesin system are closely related with renal insufficiencies having multi pathogenesis. TGF-β1 and the renin-angiotesin system are the two most critical factors in the progressive deterioration of renal disorders and the inhibition of Ang II has a close relationship with the reduction of TGF-β1. As Ang II plays an important role in the onset of various types of hypertensions, the compounds of the present invention may be used for the treatment of renal hypertensions, diabetic hypertensions, peripheral vascular hypertensions and cardio-cerebrovascular diseases having the above pathogenesis.

In vivo tests on the model of chronic renal failure induced by a 5/6 nephrectomy in rats showes that, comparing with the positive controls Benazepril and Losartan, the compounds of the present invention superiors than Benazepril and corresponds to (slightly better than) Losartan on reducing the blood serum urea nitrogen (BUN) and creatinine (Cre), as well as inhibiting TGF-β1 and Ang II.

In the tests of the renal interstitial fibrosis model from unilateral ureteral ligation in rats, the subject compound 149 is better than Benazepril and corresponds to (slightly better than) Losartan, and the pathological results show that the compound of present invention are better than Benazepril and correspond to Losartan.

The tested compounds have low toxicities, under the dosages of 5 g/kg body weights and 10 g/kg body weights, within a continuous observation of two weeks, no death in the subjected mice were observed and no other abnormal expressions were found.

In Ames test of the subjected compound 149, the negative results was obtained which shows no mutagenesis.

EXAMPLES

The various starting materials of the examples can be prepared via the ordinary method of the field or the methods commonly known by the skilled artisans, which can be prepared via e.g. the following reaction routes.

-   (1) 3-ethoxycarbonyl-7-hydroxycoumarin and     3-carboxy-7-methoxycoumarin -   (2) 3-ethoxycarbonyl-6-chloro-7-hydroxycoumarin and     3-carboxy-6-chloro-7-methoxycoumarin -   (3) 3-ethoxycarbonyl-6-ethyl-7-hydroxycoumarin and     3-carboxy-6-ethyl-7-methoxycoumarin -   (4) 3-ethoxycarbonyl-6-hexyl-7-hydroxycoumarin and     3-carboxy-6-hexyl-7-methoxycoumarin -   (5) 3-ethoxycarbonyl-6-bromo-7-hydroxycoumarin and     3-carboxy-6-bromo-7-methoxycoumarin -   (6) 3-ethoxycarbonyl-7,8-dihydroxycoumarin and     3-carboxy-7,8-dimethoxycoumarin -   (7) 3-ethoxycarbonyl-7-hydroxy-8-methylcoumarin and     3-carboxy-7-methoxy-8-methylcoumarin -   (8) 3-ethoxycarbonyl-7-hydroxy-5-methylcoumarin and     3-carboxy-7-methoxy-5-methylcoumarin -   (9) 3-ethoxycarbonyl-6-nitro-7-hydroxy-8-methylcoumarin and     3-carboxy-6-nitro-7-methoxy-8-methylcoumarin -   (10) 3-ethoxycarbonyl-6-nitro-7,8-dihydroxycoumarin and     3-carboxy-6-nitro-7,8-dimethoxycoumarin -   (11) 3-ethoxycarbonyl-5-methyl-6,8-dinitro-7-hydroxycoumarin and     3-carboxy-5-methyl-6,8-dinitro-7-hydroxy-coumarin -   (12) 3-ethoxycarbonyl-5-methyl-6,8-dinitro-7-methoxycoumarin and     3-carboxy-5-methyl-6,8-dinitro-7-methoxy-coumarin -   (13) 3-ethoxycarbonyl-5-methyl-6,8-dinitro-7-hydroxycoumarin and     3-carboxy-5-methyl-6,8-dinitro-7-hydroxy-coumarin -   (14) 3-ethoxycarbonyl-6-ethyl-7-hydroxy-8-nitrocoumarin and     3-carboxy-6-ethyl-7-hydroxy-8-nitrocoumarin -   (15) 3-ethoxycarbonyl-6-chloro-7-hydroxy-8-nitrocoumarin and     3-carboxy-6-chloro-7-hydroxy-8-nitrocoumarin -   (16) 6-carboxycoumarin -   (17) 4-carboxy-7-methylcoumarin -   (18) 4-methyl-7-carboxymethoxycoumarin -   (19) 4,8-dimethyl-7-carboxymethoxycoumarin

The following examples are intended to illustrate this invention, however these examples shall not be construed to limit the scope of the invention.

Example 1 Synthesis of 3-ethoxycarbonyl-6-chloro-7-hydroxy-8-nitro-coumarin (241)

2.75 g (10.2 mmol) of 3-ethoxycarbonyl-6-chloro-7-hydroxycoumarin was added into 10 ml of concentrated sulfuric acid, 1.74 g (20.4 mmol) of concentrated nitric acid was added in portions under the cooling of ice-salt bath, the reaction was monitored through thin-layer chromatography to confirm the completion, and ice was added to seize the reaction. The reaction mixture was then filtered and washed by water, dried to give 1.52 g of the title compound (241).

¹H-NMR 300 MHz (DMSO): 1.266 (t, 3H, CH₃), 4.232 (q, 2H, CH₂), 8.017 (s, 1H, 5-H), 8.593 (s, 1H, 4-H)

Compounds 229-246 in the tables were prepared following the same procedure.

Example 2 Synthesis of 3-ethoxycarbonyl-6-ethyl-7-hydroxy-8-nitro-coumarin (233)

Compound 233 was prepared following the preparation of compound 241, except that 3-ethoxycarbonyl-6-ethyl-7-hydroxy-coumarin was nitrated to give the title compound 233.

¹H-NMR 300 MHz (DMSO): 1.262 (t, 3H, 6-ethyl-CH₃), 1.401 (t, 3H, ester-CH₃), 2.753 (q, 2H, 6-ethyl-CH₂), 3.988 (s, 3H, 7-OCH₃), 4.408 (q, 2H, ester-CH₂), 7.527 (s, 1H, 5-H), 8.479 (s, 1H, 4-H)

Example 3 Synthesis of 3-ethoxycarbonyl-6-nitro-7,8-dimethoxy-coumarin (227)

Compound 227 was prepared following the preparation of compound 241, except that 3-ethoxycarbonyl-7,8-dimethoxycoumarin was nitrated to give the title compound 227.

¹H-NMR 300 MHz (DMSO): 1.397 (t, 3H, ester-CH₃), 4.063-4.118 (d, 6H, 7,8-OCH₃), 4.423 (q, 2H, ester-CH₂), 7.757 (s, 1H, 5-H), 9.252 (s, 1H, 4-H)

Example 4 Synthesis of 3-ethoxycarbonyl-6,8-dinitro-7-methoxy-coumarin (239)

Compound was prepared following the preparation of compound 241, except that 3-ethoxycarbonyl-7-methoxy-coumarin was bis-nitrated to give the title compound 239.

¹H-NMR 300 MHz (DMSO): 1.290 (t, 3H, ester-CH₃), 4.011 (s, 3H, 7-OCH₃), 4.292 (q, 2H, ester-CH₂), 8.873 (s, 1H, 4-H), 8.955 (s, 1H, 5-H)

Example 5 Synthesis of 3-ethoxycarbonyl-6,8-dinitro-7-hydroxy-coumarin (237)

Compound 237 was prepared following the preparation of compound 241, except that 3-ethoxycarbonyl-7-hydroxy-coumarin was bis-nitrated to give the title compound 237.

¹H-NMR 300 MHz (DMSO): 1.237 (t, 3H, ester-CH₃), 4.196 (q, 2H, ester-CH₂), 8.399 (s, 1H, 4-H), 8.636 (s, 1H, 5-H)

Example 6 Synthesis of 3-(3′-hydroxy-4′-carboxy-phenylamidocarbonyl)-6-ethyl-7-methoxy-coumarin (26)

248 mg (1 mmol) of 3-carboxy-6-ethyl-7-methoxycoumarin and 2 ml of SOCl₂ was heated to complete the reaction. After that, SOCl₂ was removed and 153 mg (1 mmol) of 4-aminosalicylic acid and 2 ml of pyridine was added. The mixture was heated to complete the reaction. The crude product was purified with DMSO to give 140 mg of the title compound (26).

¹H-NMR 300 MHz (DMSO): 1.142 (t, 3H, CH₃), 2.569 (q, 2H, CH₂), 3.906 (s, 3H, 7-OCH₃), 7.069 (d, 1H, 6′-H), 7.098 (s, 1H, 8-H), 7.509 (s, 1H, 2′-H), 7.758 (d, 1H, 5-H), 8.856 (s, 1H, 4-H), 10.848 (s, 1H, CONH), 11.399 (s, 1H, OH) Compounds 1-109, 204-206, 208, 209, 213, 214, 217, 218, 220, 222-228 in table 1 and compounds 247-249 were prepared following the same procedure.

Example 7 Synthesis of 3-(3′-carboxy-4′-hydroxy-phenylamidocarbonyl)-6-ethyl-7-methoxy-coumarin (27)

Compound 27 was prepared following the preparation of compound 26, except that 4-aminosalicylic acid was replaced by 5-amino-salicylic acid to give the title compound 27.

¹H-NMR 500 MHz (DMSO): 1.162 (t, 3H, ethyl-CH₃), 2.602 (q, 2H, ethyl-CH₂), 3.937 (s, 3H, 7-OCH₃), 6.786 (d, 1H, 5′-H), 7.178 (s, 1H, 6-H), 7.746 (d, 1H, 6′-H), 7.770 (s, 1H, 5-H), 8.239 (s, 1H, 2′-H), 8.834 (s, 1H, 4-H), 10.583 (s, 1H, CONH)

Element analysis for: C₂₀H₁₇NO₇ Calculated (%): C, 62.66; H, 4.47; N, 3.65. Found (%): C, 62.87; H, 4.49; N, 3.71.

Example 8 Synthesis of 3-(m-carboxyphenylamidocarbonyl)-7-methoxycoumarin (2)

Compound 2 was prepared following the preparation of compound 26, except that 3-carboxy-7-methoxy-coumarin reacted with m-aminobenzoic acid to give the title compound 2.

Element analysis for: C₁₈H₁₃NO₆.½H₂O Calculated (%): C, 62.07; H, 4.05; N, 4.02. Found (%): C, 62.72; H, 3.74; N, 4.55.

Example 9 Synthesis of 3-(3′-hydroxy-4′-carboxyphenylamidocarbonyl)-7-methoxy coumarin (7)

Compound 7 was prepared following the preparation of compound 26, except that 3-carboxy-7-methoxy-coumarin reacted with 4-amino-salicylic acid to give the title compound 7.

¹H-NMR 300 MHz (DMSO): 3.91 (s, 3H, 7-OCH₃), 7.08 (d, 1H, 6-H), 7.11 (s, 1H, 6′-H), 7.53 (s, 1H, 2′-H), 7.77 (d, 1H, 5-11), 7.95 (d, 1H, 5′-H), 8.91 (s, 1H, 4-H), 10.83 (s, 1H, CONH), 11.40 (br, 1H, OH)

Element analysis for: C₁₈H₁₃NO₇ Calculated (%): C, 60.85; H, 3.69; N, 3.94. Found (%): C, 60.52; H, 3.59; N, 4.10.

Example 10 Synthesis of 3-(3′-carboxy-4′-hydroxyphenylamidocarbonyl)-7-methoxy coumarin (8)

Compound 8 was prepared following the preparation of compound 26, except that 3-carboxy-7-methoxy-coumarin reacted with 5-amino-salicylic acid to give the title compound 8.

¹H-NMR 300 MHz (DMSO): 3.906 (s, 3H, 7-OCH₃), 6.964 (d, 1H, 5′-H), 7.037 (d, 1H, 6-H), 7.083 (s, 1H, 8-H), 7.745 (d, 1H, 6′-H), 8.001 (d, 1H, 5-H), 8.234 (s, 1H, 2′-H), 8.877 (s, 1H, 4-H), 10.547 (s, 1H, CONH), 11.103 (br, OH)

Element analysis for: C₁₈H₁₃NO₇ Calculated (%): C, 60.85; H, 3.69; N, 3.94. Found (%): C, 60.50; H, 3.62; N, 3.64.

Example 11 Synthesis of 3-[4′-(5″-methylisooxazol-3″-yl)-amidosulfonyl]phenylamido carbonyl]-7-methoxy-coumarin (19)

Compound 19 was prepared following the preparation of compound 26, except that 3-carboxy-7-methoxy-coumarin reacted with sulfamethoxazole (SMZ) to give the title compound 19.

Element analysis for: C₂₁H₁₇N₃O₇S.½H₂O Calculated (%): C, 54.31; H, 3.91; N, 9.05. Found (%): C, 54.56; H, 3.49; N, 8.90.

Example 12 Synthesis of 3-(3′-carboxypropylamidocarbonyl)-7-methoxycoumarin (203)

Compound 203 was prepared following the preparation of compound except that 3-carboxy-7-methoxy-coumarin reacted with γ-amino-butyric acid to give the title compound 203.

¹H-NMR 300 MHz (DMSO): 1.719 (t, 2H, 3′-CH₂), 2.235 (t, 2H, 2′-CH₂), 3.311 (t, 2H, 4′-CH₂), 3.861 (s, 3H, 7-OCH₃), 7.001 (d, 1H, 6-H), 7.074 (s, 1H, 8-H), 7.861 (d, 1H, 5-H), 8.771 (s, 1H, 4-H)

Element analysis for: C₁₅H₁₅NO₆ Calculated (%): C, 59.01; H, 4.95; N, 4.59. Found (%): C, 59.05; H, 4.60; N, 4.73.

Example 13 Synthesis of 3-[4′-(5″-methylisooxazol-3″)-amidosulfonyl]phenylamido carbonyl]-7-methoxy-8-methylcoumarin (55)

Compound 55 was prepared following the preparation of compound 26 except that 3-carboxy-7-methoxy-8-methylcoumarin reacted with SMZ to give the title compound 55.

Element analysis for: C₂₂H₁₉N₃O₇S Calculated (%): C, 56.28; H, 4.08; N, 8.95. Found (%): C, 56.61; H, 4.06; N, 9.01.

Example 14 Synthesis of 3-(m-carboxymethylenoxy-phenylamidocarbonyl)-7,8-dimethoxycoumarin (64)

Compound 64 was prepared following the preparation of compound 26 except that 3-carboxy-7,8-dimethoxy-coumarin reacted with m-carboxy methylenoxyaniline to give the title compound 64.

¹H-NMR 300 MHz (DMSO): 3.852 (s, 3H, 8-OCH₃), 3.951 (s, 3H, 7-OCH₃), 4.641 (s, 2H, OCH₂), 6.676 (q, 1H, 5′-H), 7.198-7.420 (m, 3H, 4′, 6′, 6-H), 7.502 (s, 1H, 2′-H), 7.751 (d, 1H, 5-H), 8.853 (s, 1H, 4-H), 10.584 (s, 1H, CONH)

Element analysis for: C₂₀H₁₇NO₈ Calculated (%): C, 60.15; H, 4.29; N, 3.51. Found (%): C, 60.41; H, 4.65; N, 3.75.

Example 15 Synthesis of 3-(4′-guanidinosulfonylphenylamidocarbonyl)-7,8-dimethoxy-coumarin (66)

Compound 66 was prepared following the preparation of compound 26 except that 3-carboxy-7,8-dimethoxy-coumarin reacted with sulfaguanidine (SG) to give the title compound 66.

Element analysis for: C₁₉H₁₈N₄O₇S.2H₂O Calculated (%): C, 47.30; H, 4.56; N, 11.61. Found (%): C, 47.34; H, 4.08; N, 11.00.

Example 16 Synthesis of 3-(3′-carboxy-4′-hydroxy-phenylamidocarbonyl)-7,8-dimethoxy-coumarin (60)

Compound 60 was prepared following the preparation of compound 26 except that 3-carboxy-7,8-dimethoxy-coumarin reacted with 5-amino-salicylic acid to give the title compound 60.

¹H-NMR 300 MHz (DMSO): 3.849-3.947 (d, 6H, 7,8-bis-OCH₃), 6.962 (d, 1H, 5′-H), 7.233 (d, 1H, 6-H), 7.727-7.755 (d, 2H, 5,6′-H), 8.210 (s, 1H, 2′-H), 8.813 (s, 1H, 4-H), 10.495 (s, 1H, CONH)

Element analysis for: C₁₉H₁₅NO₈.¼H₂O Calculated (%): C, 58.61; H, 4.01; N, 3.59. Found (%): C, 58.27; H, 3.86; N, 3.92.

Example 17 Synthesis of 3-(benzoylhydrazinocarbonyl)-5-methyl-7-methoxycoumarin (210)

Compound 210 was prepared following the preparation of compound 26 except that 3-carboxy-5-methyl-7-methoxy-coumarin reacted with benzoyl hydrazine to give the title compound 210.

¹H-NMR 300 MHz (DMSO): 2.482 (s, 3H, 5-CH₃), 3.888 (s, 3H, 7-OCH₃), 6.979 (d, 2H, 6, 8-H), 7.477-7.583 (q, 2H, 3′,5′-H), 7.500 (t, 1H, 5′-H), 7.889 (d, 2H, 2′,6′-H), 8.792 (s, 1H, 4-H), 10.24 (s, 1H, CON), 10.868 (s, 1H, CONH)

Example 18 Synthesis of 3-(isonicotinoylhydrazinocarbonyl)-5-methyl-7-methoxy coumarin (213)

Compound 213 was prepared following the preparation of compound 26 except that 3-carboxy-5-methyl-7-methoxy-coumarin reacted with isoniazid to give the title compound 213.

¹H-NMR 300 MHz (DMSO): 2.553 (s, 3H, 5-CH₃), 3.878 (s, 3H, 7-OCH₃), 6.979 (d, 2H, 6, 8-H), 7.935 (d, 2H, 3′,5′-H), 8.781 (s, 1H, 4-He, 10.545 (s, 1H, CONH), 11.362 (s, 1H, CONH)

Example 19 Synthesis of 3-(3′-carboxy-4′-hydroxy-phenylamidocarbonyl)-5-methyl-7-methoxycoumarin (74)

Compound 74 was prepared following the preparation of compound 26 except that 3-carboxy-5methyl-7-methoxy-coumarin reacted with 5-amino-salicylic acid to give the title compound 74.

Element analysis for: C₁₉H₁₅NO₇ Calculated (%): C, 61.79; H, 4.09; N, 3.79. Found (%): C, 61.57; H, 4.07; N, 3.81.

Example 20 Synthesis of 3-(3′-hydroxy-4′-carboxy-phenylamidocarbonyl)-6-chloro-7-methoxy-coumarin (87)

Compound 87 was prepared following the preparation of compound 26 except that 3-carboxy-6-chloro-7-methoxy-coumarin reacted with 4-amino-salicylic acid to give the title compound 87.

¹H-NMR 300 MHz (DMSO): 3.996 (s, 3H, 7-OCH₃), 7.114 (d, 1H, 6′-H), 7.376 (s, 1H, 8-H), 7.485 (s, 1H, 2′-H), 7.768 (d, 1H, 5′-H), 8.146 (s, 1H, 5-H), 8.839 (s, 1H, 4-H), 10.721 (s, 1H, CONH)

Element analysis for: C₁₈H₁₂ClNO₇ Calculated (%): C, 55.47; H, 3.11; N, 3.59. Found (%): C, 55.97; H, 3.13; N, 4.48.

Example 21 Synthesis of 3-(3′-carboxy-4′-hydroxy-phenylamidocarbonyl)-6-chloro-7-methoxy-coumarin (88)

Compound 88 was prepared following the preparation of compound 26 except that 3-carboxy-6-chloro-7-methoxy-coumarin reacted with 5-amino-salicylic acid to give the title compound 88.

¹H-NMR 300 MHz (DMSO): 4.010 (s, 3H, 7-OCH₃), 6.968 (d, 1H, 5′-H), 7.380 (s, 1H, 8-H), 7.752 (d, 1H, 6′-H), 8.153 (s, 1H, 5-H), 8.211 (s, 1H, 2′-H), 8.817 (s, 1H, 4-H), 10.475 (s, 1H, CONH)

Element analysis for: C₁₈H₁₂ClNO₇ Calculated (%): C, 55.47; H, 3.11; N, 3.59. Found (%): C, 55.60; H, 3.18; N, 4.1.

Example 22 Synthesis of 3-(3′-hydroxy-4′-carboxy-phenylamidocarbonyl)-6-bromo-7-methoxy-coumarin (96)

Compound 96 was prepared following the preparation of compound 26 except that 3-carboxy-6-bromo-7-methoxy-coumarin reacted with 4-amino-salicylic acid to give the title compound 96.

¹H-NMR 300 MHz (DMSO): 3.996 (s, 3H, 7-OCH₃), 7.118 (d, 1H, 6′-H), 7.343 (s, 1H, 8-H), 7.496 (s, 1H, 2′-H), 7.774 (d, 1H, 5′-H), 8.306 (s, 1H, 5-H), 8.846 (s, 1H, 4-H), 10.722 (s, 1H, CONH)

Example 23 Synthesis of 3-(4′-guanidinosulfonylphenylamidocarbonyl)-6-ethyl-7-methoxy-coumarin (32)

Compound 32 was prepared following the preparation of compound 26 except that 3-carboxy-6-ethyl-7-methoxy-coumarin reacted with SG to give the title compound 32.

¹H-NMR 300 MHz (DMSO): 1.148 (t, 3H, ethyl-CH₃), 2.572 (q, 2H, ethy-CH₂), 3.896 (s, 3H, OCH₃), 6.690 (br, 4H, guanidino-H), 7.125 (s, 1H, 8-H), 7.709 (s, 1H, 5-H), 7.739 (q, 4H, Ar—H), 8.827 (s, 1H, 4-H), 10.841 (s, 1H, CONH)

Element analysis for: C₂₀H₂₀N₄O₆S.¼H₂O Calculated (%): C, 53.55; H, 4.60; N, 12.48. Found (%): C, 53.49; H, 4.63; N, 12.40.

Example 24 Synthesis of 3-(4′-guanidinosulfonylphenylamidocarbonyl)-6-chloro-7-methoxy-coumarin (92)

Compound 92 was prepared following the preparation of compound 26 except that 3-carboxy-6-chloro-7-methoxy-coumarin reacted with SG to give the title compound 92.

¹H-NMR 300 MHz (DMSO): 3.999 (s, 3H, 7-OCH₃), 7.407 (s, 1H, 8-H), 7.776 (q, 4H, Ar—H), 8.172 (s, 1H, 5-H), 8.860 (s, 1H, 4-H), 10.787 (s, 1H, CONH)

Element analysis for: C₁₈H₁₅ClN₄O₆S Calculated (%): C, 47.95; H, 3.35; N, 12.43. Found (%): C, 47.54; H, 3.45; N, 12.15.

Example 25 Synthesis of 3-(3′-hydroxy-4′-carboxy-phenylamidocarbonyl)-7-methoxy-8-methyl-coumarin (43)

Compound 43 was prepared following the preparation of compound 26 except that 3-carboxy-7-methoxy-8-methyl-coumarin reacted with 4-amino-salicylic acid to give the title compound 43.

¹H-NMR 300 MHz (DMSO): 2.215 (s, 3H, 8-CH₃), 3.912 (s, 3H, 7-OCH₃), 7.081(d, 1H, 6′-H), 7.182(d, 1H, 6-H), 7.612(s, 1H, 2′-H), 7.747(d, 1H, 5-H), 7.872(d, 1H, 5′-H), 8.834(s, 1H, 4-H), 10.813(s, 1H, CONH)

Element analysis for: C₁₉H₁₅NO₇.½H₂O Calculated (%): C, 60.32; H, 4.26; N, 3.70. Found (%): C, 60.26; H, 4.03; N, 4.14.

Example 26 Synthesis of 3-(3′-carboxy-4′-hydroxy-phenylamidocarbonyl)-7-methoxy-8-methyl-coumarin (44)

Compound 44 was prepared following the preparation of compound 26 except that 3-carboxy-7-methoxy-8-methylcoumarin reacted with 5-amino-salicylic acid to give the title compound 44

¹H-NMR 300 MHz (DMSO): 2.209(s, 3H, 8-CH₃), 3.753(s, 3H, 7-OCH₃), 6.959(d, 1H, 5′-H), 7.168(d, 1H, 6-H), 7.723(d, 1H, 6′-H), 7.848(d, 1H, 5-H), 8.197(s, 1H, 2′-H), 8.794(s, 1H, 4-H), 10.504(s, 1H, CONH)

Element analysis for: C₁₉H₁₅NO₇.½H₂O Calculated (%): C, 60.32; H, 4.26; N, 3.70. Found (%): C, 59.66; H, 3.92; N, 3.81.

Example 27 Synthesis of 3-(4′-methoxy-phenylamidocarbonyl)-6-nitro-7-hydroxy-8-methyl-coumarin (146)

160 mg (0.604 mmol) of 3-carboxy-6-nitro-7-methoxy-8-methyl-coumarin and 2 ml of thionyl chloride was heated to complete the reaction. Extra thionyl chloride was removed and 74.3 mg (0.604 mmol) of p-anisidine, 1 ml of pyridine and 1 ml of DMF were added therein and the so-obtained mixture was heated to complete the reaction. The reaction mixture was then filtered and washed by water, diluted hydro chloride, water and ethanol, respectively, dried and purified with glacial acetic acid to give 170 mg of the title compound (146).

¹H-NMR 300 MHz (DMSO): 2.280(s, 3H, Ar—CH₃), 3.740(s, 3H, OCH₃), 6.941(d, 2H, 3′,5′-H), 7.621(d, 2H, 2′,6′-H), 8.673(s, 1H, 5-H), 8.897(s, 1H, 4-H), 10.374(s, 1H, CONH)

Compounds 110-203, 225-228 were prepared following the same procedure.

Example 28 Synthesis of 3-(4′-guanidinosulfonylphenylamidocarbonyl)-6-nitro-7-methoxy-8-methyl-coumarin (169)

Compound 169 was prepared following the preparation of compound 146 except that 3-carboxy-6-nitro-7-methoxy-8-methylcoumarin reacted with SG, and purified with DMF to give the title compound 169.

¹H-NMR 300 MHz (DMSO): 2.382(s, 3H, 8-CH₃), 3.940(s, 3H, 7-OCH₃), 6.677(br, 4H, guanidino-H), 7.790(q, 4H, Ar—H), 8.593(s, 1H, 5-H), 8.903(s, 1H, 4-H), 10.707(s, 1H, CONH)

Element analysis for: C₁₉H₁₇N₅O₈S.½H₂O Calculated (%): C, 47.10; H, 3.75; N, 14.46. Found (%): C, 47.27; H, 3.73; N, 14.58.

Example 29 Synthesis of 3-(4′-carboxy-phenylamidocarbonyl)-6-nitro-7,8-dimethoxy coumarin (110)

Compound 110 was prepared following the preparation of compound 146 except that 3-carboxy-6-nitro-7,8-methoxycoumarin reacted with p-amino-benzoic acid to give the title-compound 110.

¹H-NMR 300 MHz (DMSO): 3.99-4.06(q, 6H, 7,8-bis-OCH₃), 7.82(d, 2H, J=8.7, Ar—H), 7.9(d, 2H, J=8.7, Ar—H), 8.15(s, 1H, 5-H), 9.09(s, 4-H)10.91(s, 1H, CONH)

Example 30 Synthesis of 3-(3′-carboxy-phenylamidocarbonyl)-6-nitro-7,8-dimethoxy-coumarin (111)

Compound 111 was prepared following the preparation of compound 146 except that 3-carboxy-6-nitro-7,8-dimethoxycoumarin reacted with m-amino-benzoic acid to give the title compound 111.

¹H-NMR 300 MHz (DMSO): 3.97-4.05(q, 6H, 7,8-bis-OCH₃), 7.49(t, 1H, 5′-H), 7.67(d, 1H, 6′-H), 7.76(d, 1H, 4′H), 7.93(s, 1H, 2′-H), 8.32(s, 1H, 5-H), 9.08(s, 1H, 4-H), 10.66(s, 1H, CONH)

Example 31 Synthesis of 3-[4′-(5″,6″-dimethoxypyrimidine-4″)-amidosulfonyl phenylamidocarbonyl]-6-nitro-7,8-dimethoxycoumarin (123)

Compound 123 was prepared following the preparation of compound 146 except that 3-carboxy-6-nitro-7,8-methoxycoumarin reacted with sulfadoxine (SDM) to give the title compound 123.

¹H-NMR 300 MHz (DMSO): 3.694(s, 3H, pyrimidine-OCH₃), 3.894(s, 3H, 8-OCH₃), 4.064(s, 3H, 7-OCH₃), 7.886-7.996(q, 4H, Ar—H), 7.974(s, 1H, 2″-H), 8.109(s, 1H, 5-H), 9.092(s, 1H, 4-H), 10.791(s, 1H, CONH), 10.947(br, 1H, SO₂NH)

Example 32 Synthesis of 3-(3′-hydroxy-4′-carboxyphenylamidocarbonyl)-6-nitro-7-hydroxy-8-methyl-coumarin (148)

Compound 148 was prepared following the preparation of compound 146 except that 3-carboxy-6-nitro-7-hydroxy-8-methylcoumarin reacted with 4-aminosalicylic acid to give the title compound 148.

¹H-NMR 300 MHz (DMSO): 2.27(s, 3H, Ar—CH₃), 7.11(dd, 1H, J=7.8 Hz, 1.8 Hz, 6′-H), 7.498(d, 1H, J=1.8 Hz, 2′-H), 7.775(d, 1H, J=7.8, 5′-H), 8.65(s, 1H, 5-H), 8.892(s, 1H, 4-H), 10.69(s, 1H, CONH)

Example 33 Synthesis of 3-(3′-carboxy-4′-hydroxy-phenylamidocarbonyl)-6-nitro-7-hydroxy-8-methyl-coumarin (149)

Compound 149 was prepared following the preparation of compound 146 except that 3-carboxy-6-nitro-7-hydroxy-8-methylcoumarin reacted with 5-aminosalicylic acid to give the title compound 149.

¹H-NMR 300 MHz (DMSO): 2.268(s, 3H, Ar—H), 6.971(d, 1H, J=8.7 Hz, 5′-H), 7.747(dd, 1H, J=8.7 Hz, 2.7 Hz, 6′-H), 8.208(d, 1H, J=2.7 Hz, 2′-H), 8.658 (s, 1H, 5-H), 8.867(s, 1H, 4-H), 10.403(s, 1H, CONH)

Element analysis for: C₁₈H₁₂N₂O₉.½H₂O Calculated (%): C, 52.83; H, 3.22; N, 6.85. Found (%): 52.92, 3.26, 6.99.

Example 34 Synthesis of 3-[4′-(2″-pyrimidinylamidosulfonyl)phenylamidocarbonyl]-5-methyl-6,8-dinitro-7-hydroy-coumarin (200)

Compound 200 was prepared following the preparation of compound 146 except 3-carboxy-5-methyl-6,8-nitro-7-hydroxycoumarin reacted with sulfadiazine (SD) to give the title compound 200.

¹H-NMR 300 MHz (DMSO): 2.291(s, 3H, 5-CH₃), 7.025(t, 1H, 5″-H), 7.884(q, 4H, Ar—H), 8.483(d, 2H, 4″, 6″-H), 8.640(s, 1H, 4-H), 10.705(s, 1H, CONH)

Example 35 Synthesis of 3-(4′-amidosulfonylphenylamidocarbonyl)-5-methyl-6,8-dinitro-7-hydroxy-coumarin (198)

Compound 198 was prepared following the preparation of compound 146 except that 3-carboxy-5-methyl-6,8-nitro-7-hydroxycoumarin reacted with sulfanilamide to give the title compound 198.

¹H-NMR 300 MHz (DMSO): 2.254(s, 3H, 5-CH₃), 7.240(br, 2H, NH₂), 7.788(q, 4H, Ar—H), 8.666(s, 1H, 4-H), 10.676(s, 1H, CONH)

Example 36 Synthesis of 3-(2′-thiazolamidosulfonylphenylamidocarbonyl)-5-methyl-6,8-dinitro-7-hydroxy-coumarin (201)

Compound 201 was prepared following the preparation of compound 146 except that 3-carboxy-5-methyl-6,8-nitro-7-hydroxycoumarin reacted with sulfathiazole (ST) to give the title compound 201.

¹H-NMR 300 MHz (DMSO): 2.291(s, 3H, 5-CH₃), 6.802(d, 1H, thiazole-H), 7.225(d, 1H, thiazolyl-H), 7.737(q, 4H, Ar—H), 8.651(s, 1H, 4-H), 10.667(s, 1H, CONH)

Example 37 Synthesis of 3-(4′-guanidinosulfonylphenylamidocarbonyl)-5-methyl-6,8-dinitro-7-hydroxy-coumarin (199)

Compound 199 was prepared following the preparation of compound 146 except that 3-carboxy-5-methyl-6,8-dinitro-7-hydroxycoumarin reacted with SG to give the title compound 199.

¹H-NMR 300 MHz (DMSO): 2.293(s, 3H, 5-CH₃), 6.685(br, 4H, guanidino-H), 7.746(q, 4H, Ar—H), 8.657(s, 1H, 4-H), 10.647(s, 1H, CONH)

Example 38 Synthesis of 3-(2′-phenyl-1′,3′,4′-oxadiazol-5′-yl)-7-methoxy-8-methyl coumarin (216)

295 mg (0.84 mmol) of 3-(benzoylhydrazinocarbonyl)-7-methoxy-8-methylcoumarin reacted with 4.6 ml phosphorus oxychloride at 100° C. for 5 hours, and the reaction mixture was left to be cool and then poured into ice-water, filtrated, washed with water, and dried. 290 mg of the crude product was obtained, and then the crude product was purified with DMF to give 160 mg of the title compound 216.

¹H-NMR 300 MHz (DMSO): 2.252(s, 3H, 8-CH₃), 3.968(s, 3H, 7-OCH₃), 7.174(d, 1H, 6-H), 7.634(m, 3H, Ar′—H), 7.812(d, 1H, 5-H), 8.088(m, 2H, Ar′—H), 8.874(s, 1H, 4-H)

Compounds 206, 207, 210-212, 215, 216, 219 and 221 in table 2 were prepared following the same procedure.

Example 39 Synthesis of 3-(2′-phenyl-1′,3′,4′-oxadiazol-5′yl)-7-methoxycoumarin (206)

Compound 206 was prepared following the preparation of compound 216 except that 3-(benzoylhydrazinocarbonyl)-7-methoxycoumarin reacted with phosphorus oxychloride to give the title compound 206.

¹H-NMR 300 MHz (DMSO): 3.929(s, 3H, 7-OCH₃), 7.021(d, 1H, 6-H), 7.085(s, 1H, 8-H), 7.599-7.668(m, 3H, Ar—H), 7.871(d, 1H, 5-H), 8.095(m, 2H, Ar—H), 8.898(s, 1H, 4-H)

Element analysis for: C₁₈H₁₂N₂O₄ Calculated (%): C, 67.49; H, 3.78; N, 8.75. Found (%): C, 67.57; H, 3.98; N, 8.41.

Example 40 Synthesis of 3-[(2′-pyridyl-4″)-1′,3′,4′-oxadiazol-5′yl]-6-hexyl-7-methoxy coumarin (221)

Compound 221 was prepared following the preparation of compound 216 except that 3-(isonicotinoylhydrazinocarbonyl)-6-hexyl-7-methoxy coumarin reacted with phosphorus oxychloride to give the title compound 221.

¹H-NMR 300 MHz (DMSO): 0.869(t, 3H, hezyl-CH₃), 1.240(br, 6H, hezyl-CH₂), 1.574(t, 2H, hexyl-CH₂), 2.734(t, 2H, hezyl-CH₂), 3.959(s, 3H, 7-OCH₃), 7.116(s, 1H, 8-H), 7.699(s, 1H, 5-H), 8.070(br, 2H, pyridyl-H), 8.920(br, 2H, pyridyl-H), 8.921(s, 1H, 4-H)

Element analysis for: C₂₃H₂₃N₃O₄.3H₂O Calculated (%): C, 60.12; H, 6.36; N, 9.15. Found (%): C, 59.51; H, 5.51; N, 8.96.

Example 41 Synthesis of 4-methyl-7-(4′-ethoxycarbonylphenylamidocarbonyl-methylenoxy)coumarin (255)

60 mg (0.256 mmol) of 4-methyl-7-carboxy-methylenoxycoumarin and 2 ml of thionyl chloride was heated to complete the reaction. Extra thionyl chloride was removed and the residue was dissolved in 5 ml of methylene chloride. 44 mg (0.267 mmol) of ethyl 4-amino-benzoate in 5 ml methylene chloride and 3 ml of pyridine were added therein and the reaction mixture was stirred for 0.5 hours to precipitate the solid and the stirration was continued for additional 1 hour. The product was filtrated, washed with methylene chloride, and dried to give 80 mg of the title compound (255).

¹H-NMR 300 MHz (DMSO): 1.293(t, 3H, ester-methyl); 2.389(s, 3H, 4-methyl);4.269(q, 2H, ester-CH₂), 4.881(s, 2H, OCH₂), 6.219(s, 1H, 3-H), 7.018(d, 1H, 8-H), 7.056(d, 1H, 6-H), 7.712(d, 1H, 5-H), 7.760(d, 2H, 2′,6′-H), 7.919(d, 2H, 3′,5′-H), 10.479(s, 1H, CONH)

Element analysis for: C₂₁H₁₉NO₆ Calculated (%): C, 66.13; H, 5.02; N, 3.67. Found (%): C, 66.26; H, 4.91; N, 3.81.

Compounds 250-264 in table 2 were prepared following the same procedure.

Example 42 Synthesis of 4-methyl-7-phenylamidocarbonyl-methylenoxycoumarin (248)

Compound 248 was prepared following the preparation of compound 255 except that ethyl 4-amino-benzoate was replaced by aniline to give the title compound 248.

¹H-NMR 300 MHz (DMSO): 2.377(s, 3H, 4-CH₃), 4.825(s, 2H, 7OCH₂), 6.208(s, 1H, 3-H), 6.997(m, 3H, 4′, 6, 8-H), 7.306(t, 2H, 3′, 5′-H), 7.593(d, 2H, 2′,6′-H), 7.711(d, 1H, 5-H), 10, 144(s, CONH)

Element analysis for: C₁₈H₁₅NO₄ Calculated (%): C, 69.89; H, 4.89; N, 4.53. Found (%): C, 69.61; H, 4.891; N, 4.58.

Example 43 Synthesis of 4-methyl-7-(4′-carboxyphenylamidocarbonyl-methylenoxy) coumarin (252)

Compound 252 was prepared following the preparation of compound 255 except that ethyl 4-amino-benzoate was replaced by p-amino-benzoic acid to give the title compound 252.

¹H-NMR 300 MHz (DMSO): 2.404(s, 3H, 4-CH₃), 4.899(s, 2H, 7-OCH₂), 6.235(s, 1H, 3-H), 7.036(s, 1H8-H), 7.073(d, 1H, 6-H), 7.713(d, 1H, 5-H), 7.739-7.924(q, 4H, Ar—H), 10.491(s, 1H, CONH)

Element analysis for: C₁₉H₁₅NO₆.¼H₂O Calculated (%): C, 63.77; H, 4.37; N, 3.92. Found (%): C, 63.76; H, 4.28; N, 4.24.

Example 44 Synthesis of 4-methyl-7-(4′-hydroxyphenylamidocarbonyl-methylenoxy) coumarin (249)

Compound 249 was prepared following the preparation of compound 255 except that ethyl 4-amino-benzoate was replaced by p-amino-phenol to give the title compound 249.

¹H-NMR 300 MHz (DMSO): 2.084(s, 3H, 4-CH₃), 4.781(s, 2H, 7-OCH₂), 6.230(s, 1H, 3-H), 6.705-7.390(q, 4H, Ar—H), 7.014(s, 1H, 8-H), 7.060(d, 1H, 6-H), 7.723(d, 1H, 5-H), 9.905(s, 1H, CONH)

Element analysis for: C₁₈H₁₅NO₅ Calculated (%): C, 66.45; H, 4.65; N, 4.31. Found (%): C, 66.14; H, 4.62; N, 4.32.

Example 45 Synthesis of 4-methyl-7-(3′-carboxy-4′-hydroxyphenylamidocarbonyl-methylenoxy)coumarin (261)

Compound 261 was prepared following the preparation of compound 255 except that ethyl 4-amino-benzoate was replaced by 5-amino-salicylic acid to give the title compound 261.

¹H-NMR 300 MHz (DMSO): 2.495(s, 3H, 4-CH₃), 4.818(s, 2H, 7-OCH₂), 6.233(s, 1H, 3-H), 6.940(d, 1H, 6-H), 7.052(s, 1H, 8-H), 7.077(d, 1H, 5′-H),

Element analysis for: C₁₉H₁₅NO₇ Calculated (%): C, 61.79; H, 4.09; N, 3.79. Found (%): C, 61.49; H, 3.96; N, 3.86.

Example 46 Synthesis of 4-methyl-7-(3′-trifluoromethylphenylamidocarbonyl-methylenoxy)coumarin (257)

Compound 257 was prepared following the preparation of compound 255 except that ethyl 4-amino-benzoate was replaced by 3-fluoromethyl aniline to give the title compound 257.

¹H-NMR 300 MHz (DMSO): 2.389(s, 3H, 4-CH₃), 4.872(s, 2H, 7-OCH₂), 6.220(s, 1H, 3-H), 7.027-7.075(m, 2H, 6, 8-H), 7.429(d, 1H, 6′-H), 7.567(t, 1H, 5′-H), 7.719(d, 1H, 5-H), 7.857(d, 1H, 4′-H), 8.096(s, 1H, 2′-H), 10.446(s, 1H, CONH)

Element analysis for: C₁₉H₁₄F₃NO₄ Calculated (%): C, 60.48; H, 3.74; N, 3.71. Found (%): C, 60.17; H, 3.45; N, 3.79.

Example 47 Synthesis of 4-methyl-7-(3′-trifluoromethyl-4′-nitrophenylamido carbonylmethylenoxy)coumarin (258)

Compound 258 was prepared following the preparation of compound 255 except that ethyl 4-amino-benzoate was replaced by 3-fluoromethyl-4′-nitro-aniline to give the title compound 258.

¹H-NMR 300 MHz (DMSO): 2.409(s, 3H, 4-CH₃), 4.955(s, 2H, 7-OCH₂), 6.243(s, 1H, 3-H), 7.061(s, 1H, 8-H), 7.086(d, 1H, 6-H), 7.734(d, 1H, 5′-H), 8.127(d, 1H, 6′-H), 8.215(d, 1H, 5-H), 8.331(s, 1H, 2′-H), 10.945(s, 1H, CONH)

Element analysis for: C₁₉H₁₃F₃N₂O₆.½H₂O Calculated (%): C, 52.91; H, 3.27; N, 6.50. Found (%): C, 53.19; H, 3.05; N, 6.76.

Example 48 Synthesis of 4,8-dimethyl-7-(3′-trifluoromethylphenylamidocarbonyl-methylenoxy)coumarin (262)

Compound 262 was prepared following the preparation of compound 255 except that 4,8-dimethyl-7-carboxy-methylenoxycoumarin reacted with 3-fluoromethylaniline to give the title compound 262.

¹H-NMR 300 MHz (DMSO): 2.291(s, 3H, 8-CH₃), 2.392(s, 3H, 4-CH₃), 4.934(s, 2H, 7-OCH₂), 6.237(s, 1H, 3-H), 7.002(d, 1H, 6-H), 7.440(d, 1H, 6′-H), 7.564(d, 1H, 5′-H), 7.603(d, 1H, 5-H), 7.816(d, 1H, 4′-H), 8.103(s, 1H, 2′-H), 10.503(s, 1H, CONH)

Element analysis for: C₂₀H₁₆F₃NO₄ Calculated (%): C, 61.38; H, 4.12; N, 3.58. Found (%): C, 61.16; H, 4.03; N, 3.67.

Example 49 Synthesis of 4,8-dimethyl-7-(3′-hydroxy-4-carboxyphenylamidocarbonyl methylenoxy)-coumarin (264)

Compound 264 was prepared following the preparation of compound 255 except that 4,8-dimethyl-7-carboxy-methylenoxycoumarin reacted with 4-amino-salicylic acid to give the title compound 264.

¹H-NMR 300 MHz (DMSO): 2.270(s, 3H, 8-CH₃), 2.371(s, 3H, 4-CH₃), 4.931(s, 2H, 7-OCH₂), 6.215(s, 1H, 3-H), 6.958(d, 1H, 6-H), 7.087(d, 1H, 6′-H), 7.337(s, 1H, 2′-H), 7.546(d, 1H, 5′-H), 7.717(d, 1H, 5-H), 10.455(s, 1H, CONH)

Element analysis for: C₂₀H₁₇NO₇ Calculated (%): C, 62.66; H, 4.47; N, 3.65. Found (%): C, 62.43; H, 4.43; N, 3.88.

Example 50 Synthesis of 6-(4′-ethyloxycarbophenylamidocarbonyl)coumarin (265)

A mixture of 95 mg (0.5 mmol) of 6-carboxycoumarin and phosphorous pentachloride in 50 ml toluene was refluxed for 1 hour and the reaction mixture was concentrated. To the residue obtained, 83 mg (0.5 mmol) of ethyl p-amino benzoate and 1 ml of pyridine were added and the reflux was continued for additional 10 minutes. The reaction mixture was cooled down and acidified with hydrochloric acid to obtain a solid, which was purified with ethanol to give 100 mg of the title compound 265

¹H-NMR 300 MHz (DMSO): 1.31(t, 3H, ester-CH₃), 4.28(q, 2H, ester-CH₂), 6.59(d, 1H, 3-H), 7.55(d, 1H, 8-H), 7.92(d, 2H, Ar′—H), 7.96(d, 2H, Ar′—H), 8.16(m, 2H, 4, 7-H), 8.34(d, 1H, 5-H), 10.68(s, 1H, CONH)

Element analysis for: C₁₉H₁₅NO₅.½H₂O Calculated (%): C, 65.80; H, 4.65; N, 4.04. Found (%): C, 66.07; H, 4.59; N, 4.06.

Compound 266 was prepared following the same procedure.

Pharmacologic Experiments

Example 1 TGF-β-Induced Cell Growth Inhibition of the Test Compounds on Mink Pulmonary Epithelial Cells

Mink pulmonary epithelial cells were seeded in 24 well-plate at a density of 3×10⁴ cells/well and cultured in modified Eagle's medium (MEM) containing 10% fetal bovine serum in 37° C. and 5% CO₂. Next day the serum was replaced by a MEM containing 0.2% fetal bovine serum. After 24 hours, the medium was replaced with fresh medium containing 10 pmol/L TGF-β and test compounds, and incubated for 24 h. [³H]Thymidine was added to the medium 2 hours before the termination of incubation. After removing the medium, cells were washed with PBS, dissolved in 0.5 mol/L NaOH, and radioactivity was measured. The inhibitory effects of test compounds are represented as the percentage of Thymidine uptake recovery (Table 3).

Tab. 3 TGF-β-Induced Cell Growth Inhibition of Tested Compounds in Mink Lung Epithelial Cells No. of tested compounds(10 μg/ml) 26 92 73 7 2 Inhibition recovery rate 70.7 95.0 15.1 67.1 27.1 on cell growth(%)

Example 2 TGF-β Receptor Binding Antagonism Assay of Test Compounds

Balb/c 3T3 cells were seeded in 24 well-plate and cultured in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% fetal bovine serum, in 37° C. and 5% CO₂, for 2-4 days. When cells were at a near-confluent, medium was changed to the binding buffer (50 mmol/L HEPES containing NaCl, KCl, MgSO₄ and CaCl₂). The assay was initiated by addition of 50 pmol/L [¹²⁵I]TGF-β1 and test compounds. After incubation for 210-240 minutes, the medium was removed, and cells were washed with ice-cold binding buffer. Non-specific bindings in the presence of 10 nmol/L TGF-β1 were determined. The cells were then solubilized using buffer containing Triton X-100 and the radioactivity was measured (see Table 4). TABLE 4 TGF-β receptor binding antagonism of tested compounds in Balb/c 3T3 cells Compounds Inhibition IC₅₀ Compounds Inhibition IC₅₀ (10 μg/ml) Rate (%) (μg/ml) (10 μg/ml) Rate (%) μg/ml) 1 32.2 42 4.1 2 74.1 13.8 49 15.5 3 11.7 55 52.3 6 −6.0 66 52.3 7 94.2 7.8 67 16.2 9 11.4 73 60.0 12 5.1 79 16.2 14 35.9 83 21.2 21 10.1 87 91.1 22 37.4 88 111.2 5.3 25 11.6 91 34.7 26 95.4 8.5 92 106.4 27 77.2 93 29.7 31 29.2 96 82.3 33 32.1 104 42.8 34 36.4 206 −0.7 37 41.4

Example 3 Protection of Tested Compound Against Chronic Renal Failure Caused by 5/6 Nephrectomy in Rats

The model on chronic renal failure induced by partial renal ablation in rats was set up according to the Guidelines of Pre-clinic Research for New Drugs.

Male Wistar rats with initial body weights of ˜200 g, anesthetized with 35 mg/kg of pentobarbital i.p., the right kidney was removed, and the upper and lower pole parenchymas of the left kidney were resected, stop bleeding, closed the abdominal and sutured. Four weeks following surgery, the BUN, creatinine and urinary protein in rats were determined. Angiotensin II (AII) level was determined using radioimmunoassay and TGF-β1 using ELISA methods. Rats were randomly divided into seven groups, 30/group, with sham, model, Benazepril (4 mg/kg/day) and Losartan (10 mg/kg/day) as positive control, and compound 149 groups (7.5 mg/kg/day, 15 mg/kg/day and 30 mg/kg/day, respectively). All the groups were administered intragastrically, once per day and 6 (six) times per week, till 16 weeks post surgery.

Body weights were weighed to observe the growth of the rats. The above indexes were determined every 4 weeks up to the 16^(th) week following the initiation of drug administration, and at each time, a number of animals were sacrificed and the kidneys were harvested for pathological analysis.

Pathological lesions in chronic renal failure models are mainly glomerulosclerosis and interstitial fibrosis. According to the damage extents, glomerulosclerosis is divided into five grades (0˜IV). 0 grade means no glomerulus pathological changes at all, and IV grade means ultimately glomerular sclerosis and glassy pathological changes. 50 glomerulus were observed in each animal kidney tissue slide, and percentage of various grade in each group was calculated based on the above five grades.

4 weeks after nephrectomy, BUN in serum increased by 111.12% (P<0.01), urinary protein concentration increased by 86.13% (P<0.01), and TGF-β1 level increased by 70.84% (P<0.02).

12 weeks after nephrectomy (8 weeks since administration), morphological results demonstrated that the rates of 0 grade glomerylus in residual kidney in 30 mg/kg compound 149 (P<0.05) and Losartan (P<0.05) groups were higher than that of the model group with significant difference. Glomerulus pathological scores were less than that of the model group significantly. The inflammatory cell infiltration existed in some animal kidney tissues of the Benazepril group was severe, and nephrotubular enlargement as well as protein-like substance also appeared.

16 weeks after nephrectomy (12 weeks since administration), pathological results indicated that the number of grade III glomerulus in the 30 mg/kg group of compound 149 and the Losartan group was less than that of the model group significantly (P<0.01 and P<0.05, respectively). The glomerulus pathological score in the positive Benazepril group was the highest, and the inflammatory cell infiltration in kidney matrix was medium-severe, interstitial fibrosis, nephrotubular enlargement as well as protein-like substance existed.

The results are shown in Tables 5A-E. TABLE 5 Protection of test compound on chronic renal failure induced by 5/6 nephrectomy in rats A. The change of serum creatinine and BUN concentration in 8^(th) week after administration (following 12 weeks after nephrectomy) Dose Scr. Change BUN Change Groups (mg/kg) (mg/dL) Rate (%) (mg/dL) Rate (%) Sham — 2.08 ± 0.742 13.00 ± 2.326 125.90↑  Model — 3.06 ± 0.768 47.93↑  29.37 ± 3.079^(#) 28.23↑ Benazepril 4 3.54 ± 1.140 15.36↑ 37.66 ± 8.895 12.70↓ Losartan 10  2.34 ± 0.268* 23.46↓ 25.64 ± 5.116 23.89↓ Compound 7.5  2.14 ± 0.500* 30.26↓  22.35 ± 3.120* 11.33↓ 149 15  1.80 ± 0.550* 41.34↓ 26.04 ± 4.234  3.98↑ 30 1.89 ± 0.184 38.20↓  30.54 ± 11.697 B. The change of serum TGF-β1, Angiotensin II and urinary protein in 8^(th) week after administration (following 12 wk after nephrectomy) Dose TGF-β1 Change AII Change UP Change Groups (mg/kg) (ng/ml) (%) (pg/ml) (%) (mg/day) (%) Sham 20.1 ± 6.2  54.5 ± 22.7  12.7  18.3 ± 2.5  Model 46.33 ± 14.74 130.5↑  94.5 ± 7.4^(#) 73.4↑  40.7 ± 14.2^(#) 122.5↑  Benazepril 4 40.9 ± 26.6 11.72↓  74.3 ± 13.2 21.4↓ 51.1 ± 23.6  25.8↑ Losartan 10 18.7 ± 9.2   59.6↓ 96.7 ± 32.1  2.2↑ 32.7 ± 10.3  19.6↓ Compound 7.5 20.0 ± 6.7   56.8↓  63.9 ± 13.2* 32.4↓ 30.1 ± 16.6  26.0↓ 149 15 18.6 ± 12.2  59.9↓  49.9 ± 21.3* 47.2↓ 30.4 ± 16.2  25.3↓ 30 18.9 ± 10.1  59.2↓  41.0 ± 12.5* 56.6↓ 34.3 ± 12.1  15.7↓ C. The change of serum creatinine (Scr.) and BUN concentration in 12^(th) week after administration (following 16 weeks after nephrectomy) Dose Scr. Change BUN Change Groups (mg/kg) (mg/dL) Rate (%) (mg/dL) Rate (%) Sham — 2.25 ± 0.39 21.24 ± 3.354 Model —  2.71 ± 0.49^(#)  38.93 ± 8.755^(#) 83.32↑  Benazepril 4 2.28 ± 0.70 20.70↑ 39.48 ± 7.109 1.41↑ Losartan 10  2.21 ± 0.48* 37.84 ± 5.672 2.80↓ Com. 149 7.5 2.73 ± 0.78 19.01↑ 39.42 ± 4.686 1.25↑ 15 2.63 ± 0.38 37.32 ± 5.467 4.14↓ 30  2.10 ± 0.71* 22.73↓ 36.60 ± 5.422 5.99↓  0.75↓  2.87↓ 28.82↓ D. The change of serum TGF-β1, Angiotensin II and urinary protein in 12^(th) week after administration (following 16 wk after nephrectomy) Dose TGF-β1 Change AII Change UP Change Groups (mg/kg) (ng/ml) (%) (pg/ml) (%) (mg/day) (%) Sham 18.2 ± 8.9 30.0 ± 7.6  16.5 ± 17.3 Model 12.8 ± 7.9 61.7 ± 24.3 105.7↑   54.2 ± 26.1^(#)  228↑ Benazepril 4  12.8 ± 14.8 0.57↑ 47.8 ± 12.0 22.6↓ 66.3 ± 31.9 22.3↑ Losartan. 10  11.8 ± 12.6 7.48↓  38.9 ± 17.4* 37.2↑ 39.3 ± 14.2 18.4↓ Com. 149 7.5 13.6 ± 7.1 6.28↑ 48.3 ± 48.5 21.6↓ 66.7 ± 38.8 23.1↑ 15 12.3 ± 7.7 3.91↓ 41.3 ± 28.4 33.0↓ 52.3 ± 34.4 0.06↓ 30 11.6 ± 6.7 9.38↓  19.2 ± 9.19* 68.6↓ 48.2 ± 31.6 11.1↓ E. Pathological results Glomerulosclerosis Grade (%) Total wk Groups 0 I II III IV Grade 8 wk Model 10.0 ± 17.3 38.7 ± 21.2 31.5 ± 17.3 13.6 ± 18.3  1.8 ± 3.3  4.9 ± 1.5 afte Benazepril 12.2 ± 19.0 29.6 ± 23.9 28.1 ± 18.1 19.6 ± 24.2  5.9 ± 11.2 5.5 ± 2.6 ad. Losartan  54.4 ± 29.2** 31.9 ± 20.0 13.7 ± 14.0  1.1 ± 3.3** 0  2.8 ± 1.0** Com.149  30 mg/kg 28.3 ± 20.8 34.2 ± 23.0  7.5 ± 8.8* 17.5 ± 30.7  6.7 ± 13.4 4.9 ± 3.5  15 mg/kg  37.5 ± 29.3* 27.5 ± 16.3 18.8 ± 9.9  13.3 ± 20.7  2.9 ± 8.2   3.9 ± 1.7* 7.5 mg/kg 15.7 ± 19.0 24.0 ± 21.1 30.0 ± 20.8 23.2 ± 28.5  8.0 ± 13.0 5.7 ± 2.9 12 wk Model 0 3.3 ± 6.4 29.0 ± 23.4 50.5 ± 18.7 17.6 ± 14.1 8.5 ± 1.3 after Benazepril 0 0 19.1 ± 27.1 46.2 ± 15.6 34.8 ± 29.9 9.8 ± 1.4 ad. Losartan 0 10.0 ± 2.9  45.7 ± 17.7 41.9 ± 25.6  2.8 ± 4.8   7.2 ± 1.3** Com.149  30 mg/kg 0  2.0 ± 4.5   71.3 ± 11.5*  26.7 ± 7.8** 0  6.7 ± 0.2**  15 mg/kg 0  8.1 ± 14.1 38.1 ± 27.4 46.2 ± 26.1  9.1 ± 12.6 7.7 ± 1.7 7.5 mg/kg 0  2.3 ± 6.3  37.1 ± 16.0 51.4 ± 8.6  13.8 ± 20.6 8.6 ± 1.9 Note: *P < 0.05, compared with the model group; ^(#)P < 0.05, compared with the sham group; ↑increase; ↓decrease. **P < 0.01, compared with the model group.

The above various parameters with compound 149 treatment are all better than those of the Benazepril group and are equivalent to those of the Losartan group. Moreover, pathological results show that the test compound had no significant affection with the major organs such as heart, liver, spleen, and lungs.

Example 4 Inhibition of the Test Compound on Kidney Tubulointerstitial Fibrosis Caused by Unilateral Ureteral Obstructed (UUO) in Rats

Male Wistar rats with initial body weights of 180˜230 g were used. Unilateral ureteral obstruction was performed under pentobarbital anesthesia (35 mg/kg) and sterile conditions. Via a midline incision, the left ureteral was ligated. Sham surgery was performed by making a midline incision but leaving ureteral intact. Following surgery, rats were randomly divided into sham, model, Benazepril (4 mg/kg/day) and Losartan (10 mg/kg/day) as positive control, and compound 149 (5 mg/kg/day, 10 mg/kg/day and 20 mg/kg/day). Starting 2 day before surgery, Benazepril, Losartan and compound 149 were administered for 16 days orally. BUN and creatinine in serum were determined in the 11^(th) and 16^(th) day (Table 6) following initiation of Benazepril, Losartan and compound 149 treatment, at which time animals were killed and the kidneys were harvested. Tissues were dissected, weighed, fixed in 10% formaldehyde and embedded in paraffin wax for pathological analysis. The 9^(th) day serum BUN and creatinine in model group after surgery increased 78.7% (P<0.01) and 20.73% (P<0.05) respectively. TABLE 6 Inhibition of test compound on kidney tubulointerstitial fibrosis caused by unilateral ureteral obstruction (UUO) in rats Change Change Dose Scr. Rate BUN Rate Groups (mg/kg) (mg/kg) (%) (mg/dL) (%) Sham — 1.45 ± 0.44 16.23 ± 2.70 Model —  2.20 ± 0.14^(#) 51.58↑  27.54 ± 3.32^(#) 69.73↑ Benazepril  4.0 1.92 ± 0.29 12.50↓  20.99 ± 1.58* 23.78↓ Losartan 10.0 2.15 ± 0.51  2.31↓ 23.88 ± 2.94 13.30↓ Com.149  5.0  1.58 ± 0.49* 28.24↓ 23.71 ± 4.17 13.92↓ 10.0  1.61 ± 0.36* 26.50↓  20.76 ± 1.56* 24.61↓ 20.0  1.60 ± 0.14* 27.27↓  20.77 ± 2.04* 24.58↓ Note: *P < 0.05, compared with model group; ^(#)P < 0.05, compared with control group; ↑increase, ↓decrease.

In this assay, the improvement of each biochemical index with compound 149 treatment are more significant than those of the Losartan group, and equivalent to those of the Benazepril group. There was slight different in the pathological changes: the inflammatory cell infiltration in the Benazepril group was more significant, and 4/7 of the animals had focal abscess formations in the medulla of kidney, many kidney cell necrosis and inflammatory cells and abscess cells overlapped in the Benazepril group. The inflammatory cell infiltration and tubulointerstitial fibrosis were significantly attenuated in both compound 149 and the Losartan groups. That is to say, compound 149 is better than Benazepril and equivalent with Losartan in the pathological results.

Example 5 Primary Acute Toxicity Test for Test Compound

5 g/kg and 10 g/kg of compound 149 were administered orally to mice once and observed for 14 days. Body weights of 48 hours after administration in mice were no different. At 14^(th) day after administration, the mouse average body weights in 5 g/kg and 10 g/kg groups increased 7 g and 5 g respectively. There was no any other different for every animal and no death were observed.

Example 6 Ames Test

His⁻ type Salmonella typhimurium TA97, TA98, TA100 and TA102 were employed. Concentration of the test compound was 0.5, 5.0, 50.0, 500.0, 5000.0 μg/plate. S9 was the microsome component of liver homogenate of a rat weighted 200 g. The test compound 149 was tested in the presence or absence of S9.

According to the Salmonella typhimurium/mammalian microsome enzyme mutagenic test method revised by Ames (1983), metabolism activated or non-metabolism activated plate incorporation assay was conducted on compound 149, and the strain which passed the assay was seeded to the culture medium and incubated at 37° C. under shaking for 15 hours. 100 μl compound solutions with various concentrations were added to 0.1 ml of the culture liquid, and then S9 mixture or phosphate buffer was added, and the mixtures were incubated in a 37° C. water bath for 20 minutes. After that, 2 ml of upper layer agar was added, mixed well and poured into a plate with lower layer agar and incubated at 37° C. for 48 hours. The number of the colonies in each plate was counted.

The results show that the number of colony formation of Salmonella Typhimurium TA97, TA98, TA100 and TA102 induced by compound 149 did not increase. It suggests that compound 149 has no mutagenesis. 

1. A compound represented by the following general formula (I)

characterized in that R³ is selected from the group consisting of H, carboxyl, alkyloxycarbonyl, 5′-(phenyloxadiazol-2′-yl), 5′-(pyridyl-4″-oxadizol-2′-yl),

CONHR₉, wherein R₉ is selected from the group consisting of C₂-C₈ fatty acid, benzoxamido, isonicotinamido, un-substituted or mono- or multi-substituted phenyl wherein the substituent may be hydroxyl, C₁-C₈ alkoxyl, CF₃, carboxyl, alkyloxycarbonyl, OCH₂CO₂H, NO₂, halogen, SO₃H, SO₂NHR₁₁, wherein R₁₁ is selected from the group consisting of hydrogen, amidino, 2″-thiazolyl, 3″-(5″-methylisooxazolyl), 2″-pyrimidinyl, 2″-(4″,6″-dimethylpyrimidinyl), 4″-(5″,6″-dimethoxypyrimidinyl); R₄ is selected from the group consisting of hydrogen, CONHR₁₀, wherein R₁₀ is selected from the group consisting of C₂-C₈ fatty acid, benzoxamido, isonicotiniamido, un-substituted, mono- or multi-substituted phenyl wherein the substituent may be hydroxyl, C₁-C₈ alkoxyl, CF₃, carboxyl, alkoxycarbonyl, OCH₂CO₂H, NO₂, halogen, SO₃H, SO₂NHR₁₂, wherein R₁₂ is selected from the group consisting of H, amidino, 2″-thiazolyl, 3″-(5″-methylisooxazolyl), 2″-pyrimidinyl, 2″-(4″,6″-dimethyl-pyrimidinyl), 4″-(5″,6″-dimethoxy pyrimidinyl); R₅ is selected from the group consisting of H, C₁-C₄ alkyl; R₆ is selected from the group consisting of H, C₁-C₁₂ alkyl, halogen, NO₂, CONHR₁₃, wherein R₁₃ is substituted phenyl; R₇ is selected from the group consisting of H, hydroxyl, C₁-C₄ alkyl or alkoxyl, carboxylalkylenoxyl, OCH₂CONHR₁₄, wherein R₁₄ is selected from the group consisting of un-substituted, mono- or multi-substituted phenyl wherein the substituent may be hydroxyl, OCH₃, CF₃, CO₂H, CO₂C₂H₅, NO₂; R₈ is selected from the group consisting of H, C₁-C₄ alkyl or alkoxyl, NO₂; provided that, in case that R₃, R₅ and R₅ are H and R₇ is OH, R₄ and R₇ are not groups selected from H, C₁₋₆ alkyl or C₁₋₆ alkoxy.
 2. The compound according to claim 1, characterized in that R₃ is selected from the group consisting of H, COOH, CO₂C₂H₅, 5′-(phenyloxadiazol-2′-yl), 5′-(pyridyl-4″-oxadizol-2′)-yl,

CONHR₉, wherein R₉ is n-butyric acid, o-, m-, p-phenol, o-, m-, p-carboxyl-phenyl, o-, m-, p-alkyloxycarbophenyl, methoxylphenyl, 3′-hydroxy-4′-carboxyphenyl, 3′-salicylyl, 4′-salicylyl, m-CF₃-phenyl, 3′-CF₃-4′-NO₂-phenyl, 2′-CO₂H-4′-I-phenyl, isonicotinamido, benzoxamido, 3′-carboxy-methylenoxyphenyl, 4′-amidosulfonylphenyl, 4′-guanidinosulfonylphenyl, 4′-(2″-thiazolamidosulfonyl)phenyl, 4′-(5″-methylisooxazolyl-3″-amidosulfonyl)phenyl, 4′-(pyrimidinyl-2″-amidosulfonyl)phenyl, 4′-(4″,6″-dimethylpyrimidinyl-2″-amidosulfonyl)phenyl, 4′-(5″, 6″-dimethoxypyrimidinyl-4″-amidosulfonyl)phenyl; R₄ is selected from the group consisting of H, CONHR₁₀, wherein R₁₀ is selected from the group consisting of H, 4′-CO₂H-phenyl, 4′-CO₂C₂H₅phenyl, 3′-CF₃-phenyl; R₅ is selected from the group consisting of H, CH₃; R₆ is selected from the group consisting of H, C₂H₅, n-C₆H₁₃, NO₂, NH₂, Cl, Br, CONHR₁₃, wherein R₁₃ is selected from the group consisting of 4-benzoic acid and ethyl 4-benzoate; R₇ is selected from the group consisting of H, OH, CH₃, OCH₃, OCH₂CONHR₁₄, wherein R₁₄ is selected from the group consisting of phenyl, o-, m- and p-hydroxyphenol, o-, m- and p-carboxylphenyl, m- and p-ethoxycarbonylphenyl, m-CF₃-phenyl, m-CF₃-p-NO₂-phenyl, p-CH₃O-phenyl, 4-salicylyl, 3-salicylyl; R₈ is selected from the group consisting of H, CH₃, OCH₃, NO₂; provided that, in case that R₃, R₅ and R₅ are H and R₇ is OH, R₄ and R₇ are not groups selected from H, C₁₋₆ alkyl or C₁₋₆ alkoxy.
 3. The compound according to claim 1, characterized in that the compound is represented by the following general formula (Ia)

wherein R₄, R₅, R₆, R₇, R₈ are as defined in claim 1,


4. The compound according to claim 1, characterized in that the compound is represented by the following general formula (Ib)

wherein R₄, R₅, R₆, R₇, R₈, are as defined in claim 1, R′₂ is selected from the group consisting of H, OH, CO₂H, R′₃ is selected from the group consisting of H, OH, CO₂H, CF₃, OCH₂CO₂H, R′₄ is selected from the group consisting of H, OH, CO₂H, CO₂Et, iodo, NO₂, OCH₃, SO₃H, SO₂NH₂, SONH(C═NH)NH₂,

R′₅, R′₆ are each H.
 5. The compound according to claim 2, characterized in that R₃, R₄, R₅, R₆, R₇, R₈ are respectively selected from one of the combinations in the following group consisting of: R₃=p-CO₂H-phenylamidocarbonyl, R₄=R₅=R₆=R₈=H, R₇=OCH₃; R₃=m-CO₂H-phenylamidocarbonyl, R₄=R₅=R₆=R₈=H, R₇=OCH₃; R₃=o-CO₂H-phenylamidocarbonyl, R₄=R₅=R₆=R₈=H, R₇=OCH₃; R₃=o-OH-phenylamidocarbonyl, R₄=R₅=R₆=R₈=H, R₇=OCH₃; R₃=m-OH-phenylamidocarbonyl, R₄=R₅=R₆=R₈=H, R₇=OCH₃; R₃=p-OH-phenylamidocarbonyl, R₄=R₅=R₆=R₈=H, R₇=OCH₃; R₃=m-OH-p-CO₂H-phenylamidocarbonyl, R₄=R₅=R₆=R₈=H, R₇=OCH₃; R₃=m-CO₂H-p-OH-phenylamidocarbonyl, R₄=R₅=R₆=R₈=H, R₇=OCH₃; R₃=o-CO₂H-p-I-phenylamidocarbonyl, R₄=R₅=R₆=R₈=H, R₇=OCH₃; R₃=4′-ethoxycarbonylphenylamidocarbonyl, R₄=R₅=R₆=R₈=H, R₇=OCH₃; R₃=m-CF₃-phenylamidocarbonyl, R₄=R₅=R₆=R₈=H, R₇=OCH₃; R₃=m-CF₃-p-NO₂-phenylamidocarbonyl, R₄=R₅=R₆=R₈=H, R₇=OCH₃; R₃=4′-amidosulfonylphenylamidocarbonyl, R₄=R₅=R₆=R₈=H, R₇=OCH₃; R₃=4′-guanidinosulfonylphenylamidocarbonyl, R₄=R₅=R₆=R₈=H, R₇=OCH₃; R₃=4′-(2″-thiazolamidosulfonyl)phenylamidocarbonyl, R₄=R₅=R₆=R₈=H, R₇=OCH₃; R₃=4′-(2″-pyrimidinylamidosulfonyl)phenylamidocarbonyl, R₄=R₅=R₆=R₈=H, R₇=OCH₃; R₃=4′-[2″-(4″,6″-dimethylpyrimidinylamidosulfonyl)]henylamidocarbonyl, R₄=R₅=R₆=R₈=H, R₇=OCH₃; R₃=4′-(5″,6″-dimethoxypyrimidinyl-4″-amidosulfonyl)phenylamidocarbonyl, R₄=R₅=R₆=R₈=H, R₇=OCH₃; R₃=4′-(5″-methyl-isooxazol-3″-amidosulfonyl)phenylamidocarbonyl, R₄=R₅=R₆=R₈=H, R₇=OCH₃; R₃=p-OCH₃-phenylamidocarbonyl, R₄=R₅=R₆=R₈=H, R₇=OCH₃; R₃=p-SO₃H-phenylamidocarbonyl, R₄=R₅=R₆=R₈=H, R₇=OCH₃; R₃=p-CO₂H-phenylamidocarbonyl, R₄=R₅=R₈=H, R₆=C₂H₅, R₇=OCH₃; R₃=m-CO₂H-phenylamidocarbonyl, R₄=R₅=R₈=H, R₆=C₂H₅, R₇=OCH₃; R₃=o-CO₂H-phenylamidocarbonyl, R₄=R₅=R₈=H, R₆ =C ₂H₅, R₇=OCH₃; R₃=p-OH-phenylamidocarbonyl, R₄=R₅=R₈=H, R₆=C₂H₅, R₇=OCH₃; R₃=m-OH-p-CO₂H-phenylamidocarbonyl, R₄=R₅=R₈=H, R₆=C₂H₅, R₇=OCH₃; R₃=m-CO₂H-p-OH-phenylamidocarbonyl, R₄=R₅=R₈=H, R₆=C₂H₅, R₇=OCH₃; R₃=4′-ethoxycarbonylphenylamidocarbonyl, R₄=R₅=R₈=H, R₆=C₂H₅, R₇=OCH₃; R₃=m-CF₃-phenylamidocarbonyl, R₄=R₅=R₈=H, R₆=C₂H₅, R₇=OCH₃; R₃=m-CF₃-4-NO₂-phenylamidocarbonyl, R₄=R₅=R₈=H, R₆=C₂H₅, R₇=OCH₃; R₃=4′-amidosulfonylphenylamidocarbonyl, R₄=R₅=R₈=H, R₆=C₂H₅, R₇=OCH₃; R₃=4′-guanidinosulfonylphenylamidocarbonyl, R₄=R₅=R₈=H, R₆=C₂H₅, R₇=OCH₃; R₃=4′-(2″-thiazolamidosulfonyl)phenylamidocarbonyl, R₄=R₅=R₈=H, R₆=C₂H₅, R₇=OCH₃; R₃=4′-(2″-pyrimidinylamidosulfonyl)phenylamidocarbonyl, R₄=R₅=R₈=H, R₆=C₂H₅, R₇=OCH₃; R₃=4′-(4″,6″-dimethylpyrimidinyl-2′-amidosulfonyl)henylamidocarbonyl, R₄=R₅=R₈=H, R₆=C₂H₅, R₇=OCH₃; R₃=4′-(5″,6″-dimethoxypyrimidinyl-4″-amidosulfonyl)henylamidocarbonyl, R₄=R₅=R₈=H, R₆=C₂H₅, R₇=OCH₃; R₃=4′-(5″-CH₃-isooxazol-3″-amidosulfonyl)phenylamidocarbonyl, R₄=R₅=R₈=H, R₆=C₂H₅, R₇=OCH₃; R₃=p-OCH₃-phenylamidocarbonyl, R₄=R₅=R₈=H, R₆=C₂H₅, R₇=OCH₃; R₃=p-SO₃H-phenylamidocarbonyl, R₄=R₅=R₈=H, R₆=C₂H₅, R₇=OCH₃; R₃=p-CO₂H-phenylamidocarbonyl, R₄=R₅=R₆=H, R₇=OCH₃, R₈=CH₃; R₃=m-CO₂H-phenylamidocarbonyl, R₄=R₅=R₆=H, R₇=OCH₃, R₈=CH₃; R₃=o-CO₂H-phenylamidocarbonyl, R₄=R₅=R₆=H, R₇=OCH₃, R₈=CH₃; R₃=m-OH-p-CO₂H-phenylamidocarbonyl, R₄=R₅=R₆=H, R₇=OCH₃, R₈=CH₃; R₃=m-CO₂H-p-OH-phenylamidocarbonyl, R₄=R₅=R₆=H, R₇=OCH₃, R₈=CH₃; R₃=o-CO₂H-p-I-phenylamidocarbonyl, R₄=R₅=R₆=H, R₇=OCH₃, R₈=CH₃; R₃=p-ethoxycarbophenylamidocarbonyl, R₄=R₅=R₆=H, R₇=OCH₃, R₈=CH₃; R₃=m-CF₃-phenylamidocarbonyl, R₄=R₅=R₆=H, R₇=OCH₃, R₈=CH₃; R₃=m-CF₃-4-NO₂-phenylamidocarbonyl, R₄=R₅=R₆=H, R₇=OCH₃, R₈=CH₃; R₃=4′-amidosulfonylphenylamidocarbonyl, R₄=R₅=R₆=H, R₇=OCH₃, R₈=CH₃; R₃=4′-guanidinosulfonylphenylamidocarbonyl, R₄=R₅=R₆=H, R₇=OCH₃, R₈=CH₃; R₃=4′-(2″-thiazolamidosulfonyl)phenylamidocarbonyl, R₄=R₅=R₆=H, R₇=OCH₃, R₈=CH₃; R₃=4′-(2″-pyrimidinylamidosulfonyl)phenylamidocarbonyl, R₄=R₅=R₆=H, R₇=OCH₃, R₈=CH₃; R₃=4′-(4″,6″-dimethylpyrimidinyl-2″-amidosulfonyl)henylamidocarbonyl, R₄=R₅=R₆=H, R₇=OCH₃, R₈=CH₃; R₃=4′-(5″,6″-dimethoxypyrimidinyl-4″-amidosulfonyl)henylamidocarbonyl, R₄=R₅=R₆=H, R₇=OCH₃, R₈=CH₃; R₃=4′-(5″-CH₃-isooxazol-3″-amidosulfonyl)phenylamidocarbonyl, R₄=R₅=R₆=H, R₇=OCH₃, R₈=CH₃; R₃=p-OCH₃-phenylamidocarbonyl, R₄=R₅=R₆=H, R₇=OCH₃, R₈=CH₃; R₃=p-SO₃H-phenylamidocarbonyl, R₄=R₅=R₆=H, R₇=OCH₃ R₈=CH₃; R₃=p-CO₂H-phenylamidocarbonyl, R₄=R₅=R₆=H, R₇=R₈=OCH₃; R₃=m-OH-p-CO₂H-phenylamidocarbonyl, R₄=R₅=R₆=H, R₇=R₈=OCH₃; R₃=m-CO₂H-p-OH-phenylamidocarbonyl, R₄=R₅=R₆=H, R₇=R₈=OCH₃; R₃=p-ethoxycarbophenylamidocarbonyl, R₄=R₅=R₆=H, R₇=R₈=OCH₃; R₃=m-CF₃-phenylamidocarbonyl, R₄=R₅=R₆=H, R₇=R₈=OCH₃; R₃=m-CF₃-p-NO₂-phenylamidocarbonyl, R₄=R₅=R₆=H, R₇=R₈=OCH₃; R₃=m-HO₂CCH₂O-phenylamidocarbonyl, R₄=R₅=R₆=H, R₇=R₈=OCH₃; R₃=4′-amidosulfonylphenylamidocarbonyl, R₄=R₅=R₆=H, R₇=R₈=OCH₃; R₃=4′-guanidinosulfonylphenylamidocarbonyl, R₄=R₅=R₆=H, R₇=R₈=OCH₃; R₃=p-CO₂H-phenylamidocarbonyl, R₄=R₆=R₈=H, R₅=CH₃, R₇=OCH₃; R₃=m-CO₂H-phenylamidocarbonyl, R₄=R₆=R₈=H, R₅=CH₃, R₇=OCH₃; R₃=o-CO₂H-phenylamidocarbonyl, R₄=R₆=R₈=H, R₅=CH₃, R₇=OCH₃; R₃=o-OH-phenylamidocarbonyl, R₄=R₆=R₈=H, R₅=CH₃, R₇=OCH₃; R₃=m-OH-phenylamidocarbonyl, R₄=R₆=R₈=H, R₅=CH₃, R₇=OCH₃; R₃=p-OH-phenylamidocarbonyl, R₄=R₆=R₈=H, R₅=CH₃, R₇=OCH₃; R₃=m-OH-p-CO₂H-phenylamidocarbonyl, R₄=R₆=R₈=H, R₅=CH₃ R₇=OCH₃; R₃=m-CO₂H-p-OH-phenylamidocarbonyl, R₄=R₆=R₈=H, R₅=CH₃, R₇=OCH₃; R₃=p-ethoxycarbophenylamidocarbonyl, R₄=R₆=R₈=H, R₅=CH₃, R₇=OCH₃; R₃=m-CF₃-phenylamidocarbonyl, R₄=R₆=R₈=H, R₅=CH₃, R₇=OCH₃; R₃=m-CF₃-p-NO₂-phenylamidocarbonyl, R₄=R₆=R₈=H, R₅=CH₃, R₇=OCH₃; R₃=4′-amidosulfonylphenylamidocarbonyl, R₄=R₆=R₈=H, R₅=CH₃, R₇=OCH₃; R₃=4′-guanidinosulfonylphenylamidocarbonyl, R₄=R₆=R₈=H, R₅=CH₃, R₇=OCH₃; R₃=4′-(2″-thiazolamidosulfonyl)phenylamidocarbonyl, R₄=R₆=R₈=H, R₅=CH₃, R₇-OCH₃; R₃=4′-(2″-pyrimidinylamidosulfonyl)phenylamidocarbonyl, R₄=R₆=R₈=H, R₅=CH₃, R₇=OCH₃; R₃=4′-(4″,6″-dimethylpyrimidinyl-2″-amidosulfonyl) phenylamidocarbonyl, R₄=R₆=R₈=H, R₅=CH₃, R₇=OCH₃; R₃=4′-(5″,6″-dimethoxypyrimidinyl-4″-amidosulfonyl) phenylamidocarbonyl, R₄=R₆=R₈=H, R₅=CH₃, R₇=OCH₃; R₃=4′-(5″-CH₃-isooxazol-3″-amidosulfonyl)phenylamidocarbonyl, R₄=R₆=R₈=H, R₅=CH₃, R₇=OCH₃; R₃=p-OCH₃-phenylamidocarbonyl, R₄=R₆=R₈=H, R₅=CH₃, R₇=OCH₃; R₃=p-CO₂H-phenylamidocarbonyl, R₄=R₅=R₈=H, R₆=Cl, R₇=OCH₃; R₃=m-OH-p-CO₂H-phenylamidocarbonyl, R₄=R₅=R₈=H, R₆=Cl, R₇=OCH₃; R₃=m-CO₂H-p-OH-phenylamidocarbonyl, R₄=R₅=R₈=H, R₆=Cl, R₇=OCH₃; R₃=p-ethoxycarbophenylamidocarbonyl, R₄=R₅=R₈=H, R₆=Cl, R₇=OCH₃; R₃=m-CF₃-phenylamidocarbonyl, R₄=R₅=R₈=H, R₆=Cl, R₇=OCH₃; R₃=4′-amidosufonylphenylamidocarbonyl, R₄=R₅=R₈=H, R₆=Cl, R₇=OCH₃; R₃=′4-guanidinosulfonylphenylamidocarbonyl, R₄=R₅=R₈=H, R₆=Cl, R₇=OCH₃; R₃=4′-(5″,6″-dimethoxypyrimidinyl-4″-amidosulfonyl) phenylamidocarbonyl, R₄=R₅=R₈=H, R₆=Cl, R₇=OCH₃; R₃=p-CO₂H-phenylamidocarbonyl, R₄=R₅=R₈=H, R₆=Br, R₇=OCH₃; R₃=o-CO₂H-phenylamidocarbonyl, R₄=R₅=R₈=H, R₆=Br, R₇=OCH₃; R₃=m-OH-p-CO₂H-phenylamidocarbonyl, R₄=R₅=R₈=H, R₆=Br, R₇=OCH₃; R₃=o-CO₂H-p-I-phenylamidocarbonyl, R₄=R₅=R₈=H, R₆=Br, R₇=OCH₃; R₃=p-ethoxycarbophenylamidocarbonyl, R₄=R₅=R₈=H, R₆=Br, R₇=OCH₃; R₃=m-CF₃-phenylamidocarbonyl, R₄=R₅=R₈=H, R₆=Br, R₇=OCH₃; R₃=4′-amidosufonylphenylamidocarbonyl, R₄=R₅=R₈=H, R₆=Br, R₇=OCH₃; R₃=p-OCH₃-phenylamidocarbonyl, R₄=R₅=R₈=H, R₆=Br, R₇=OCH₃; R₃=p-CO₂H-phenylamidocarbonyl, R₄=R₅=R₈=H, R₆=n-Hex, R₇=OCH₃; R₃=o-CO₂H-phenylamidocarbonyl, R₄=R₅=R₈=H, R₆=n-Hex, R₇=OCH₃; R₃=m-OH-p-CO₂H-phenylamidocarbonyl, R₄=R₅=R₈=H, R=Hex, R₇=OCH₃; R₃=o-CO₂H-p-I-phenylamidocarbonyl, R₄=R₅=R₈=H, R₆=n-Hex, R₇=OCH₃; R₃=p-ethoxycarbophenylamidocarbonyl, R₄=R₅=R₈=H, R₆=Hex, R₇=OCH₃; R₃=m-CF₃-phenylamidocarbonyl, R₄=R₅=R₈=H, R₆=Hexyl, R₇=OCH₃; R₃=4′-amidosulfonylphenylamidocarbonyl, R₄=R₅=R₈=H, R₆=Hex, R₇=OCH₃; R₃=p-OCH₃-phenylamidocarbonyl, R₄=R₅=R₈=H, R₆=Hex, R₇=OCH₃; R₃=p-CO₂H-phenylamidocarbonyl, R₄=R₅=H, R₆=NO₂, R₇=R₈=OCH₃; R₃=m-CO₂H-phenylamidocarbonyl, R₄=R₅=H, R₆=NO₂, R₇=R₈=OCH₃; R₃=p-OCH₃-phenylamidocarbonyl, R₄=R₅=H, R₆=NO₂, R₇=R₈=OCH₃; R₃=m-OH-phenylamidocarbonyl, R₄=R₅=H, R₆=NO₂, R₇=R₈=OCH₃; R₃=o-OH-phenylamidocarbonyl, R₄=R₅=H, R₆=NO₂, R₇=R₈=OCH₃; R₃=p-ethoxycarbophenylamidocarbonyl, R₄=R₅=H, R₆=NO₂, R₇=R₈=OCH₃; R₃=m-OH-p-CO₂H-phenylamidocarbonyl, R₄=R₅=H, R₆=NO₂, R₇=R₈=OCH₃; R₃=m-CO₂H-p-OH-phenylamidocarbonyl, R₄=R₅=H, R₆=NO₂, R₇=R₈=OCH₃; R₃=m-CF₃-phenylamidocarbonyl, R₄=R₅=H, R₆=NO₂, R₇=R₈=OCH₃; R₃=m-CF₃-p-NO₂-phenylamidocarbonyl, R₄=R₅=H, R₆=NO₂, R₇=R₈=OCH₃; R₃=4′-amidosufonylphenylamidocarbonyl, R₄=R₅=H, R₆=NO₂, R₇=R₈=OCH₃; R₃=4′-guanidinosulfonylphenylamidocarbonyl, R₄=R₅=H, R₆=NO₂, R₇=R₈=OCH₃; R₃=4′-(2″-pyrimidinylamidosulfonyl)phenylamidocarbonyl, R₄=R₅=H, R₆=NO₂, R₇=R₈=OCH₃; R₃=4′-(5″,6″-dimethoxypyrimidinyl-4″-amidosulfonyl) phenylamidocarbonyl, R₄=R₅=H, R₆=NO₂, R₇=R₈=OCH₃; R₃=4′-(2″-thiazolamidosulfonyl)phenylamidocarbonyl, R₄=R₅=H, R₆=NO₂, R₇=R₈=OCH₃; R₃=p-CO₂H-phenylamidocarbonyl, R₄=R₅=H, R₆=C₂H₅, R₇=OH, R₈=NO₂; R₃=p-OCH₃-phenylamidocarbonyl, R₄=R₅=H, R₆=C₂H₅, R₇=OH, R₈=NO₂; R₃=m-OH-phenylamidocarbonyl, R₄=R₅=H, R₆=C₂H₅, R₇=OH, R₈=NO₂; R₃=o-OH-phenylamidocarbonyl, R₄=R₅=H, R₆=C₂H₅, R₇=OH, R₈=NO₂; R₃=p-ethoxycarbophenylamidocarbonyl, R₄=R₅=H, R₆=C₂H₅, R₇=OH, R₈=NO₂; R₃=m-OH-p-CO₂H-phenylamidocarbonyl, R₄=R₅=H, R₆=C₂H₅, R₇=OH, R₈=NO₂; R₃=m-CO₂H-p-OH-phenylamidocarbonyl, R₄=R₅=H, R₆=C₂H₅, R₇=OH, R₈=NO₂; R₃=m-CF₃-phenylamidocarbonyl, R₄=R₅=H, R₆=C₂H₅, R₇=OH, R₈=NO₂; R₃=4′-amidosulfonylphenylamidocarbonyl, R₄=R₅=H, R₆=C₂H₅, R₇=OH, R₈=NO₂; R₃=4′-guanidinosulfonylphenylamidocarbonyl, R₄=R₅=H, R₆=C₂H₅, R₇=OH, R₈=NO₂; R₃=4′-(2″-thiazolamidosulfonyl)phenylamidocarbonyl, R₄=R₅=H, R₆=C₂H₅, R₇=OH, R₈=NO₂; R₃=p-CO₂H-phenylamidocarbonyl, R₄=R₅=H, R₆=C₂H₅, R₇=OCH₃, R₈=NO₂; R₃=p-OH-phenylamidocarbonyl, R₄=R₅=H, R₆=C₂H₅, R₇=OCH₃, R₈=NO₂; R₃=p-OCH₃-phenylamidocarbonyl, R₄=R₅=H, R₆=C₂H₅, R₇=OCH₃, R₈=NO₂; R₃=p-ethoxycarbophenylamidocarbonyl, R₄=R₅=H, R₆=C₂H₅, R₇=OH, R₈=NO₂; R₃=4′-guanidinosulfonylphenylamidocarbonyl, R₄=R₅=H, R₆=C₂H₅, R₇=OCH₃, R₈=NO₂; R₃=p-CO₂H-phenylamidocarbonyl, R₄=R₅=H, R₆=NO₂, R₇=OH, R₈=CH₃; R₃=o-CO₂H-phenylamidocarbonyl, R₄=R₅=H, R₆=NO₂, R₇=OH, R₈=CH₃; R₃=p-OH-phenylamidocarbonyl, R₄=R₅=H, R₆=NO₂, R₇=OH, R₈=CH₃; R₃=m-OH-phenylamidocarbonyl, R₄=R₅=H, R₆=NO₂, R₇=OH, R₈=CH₃; R₃=o-OH-phenylamidocarbonyl, R₄=R₅=H, R₆=NO₂, R₇=OH, R₈=CH₃; R₃=p-OCH₃-phenylamidocarbonyl, R₄=R₅=H, R₆=NO₂, R₇=OH, R₈=CH₃; R₃=p-ethoxycarbophenylamidocarbonyl, R₄=R₅=H, R₆=NO₂, R₇=OH, R₈=CH₃; R₃=m-OH-p-CO₂H-phenylamidocarbonyl, R₄=R₅=H, R₆=NO₂, R₇=OH, R₈=CH₃; R₃=m-CO₂H-p-OH-phenylamidocarbonyl, R₄=R₅=H, R₆=NO₂, R₇=OH, R₈=CH₃ R₃=m-CF₃-phenylamidocarbonyl, R₄=R₅=H, R₆=NO₂, R₇=OH, R₈=CH₃ R₃=m-CF₃-p-NO₂-phenylamidocarbonyl, R₄=R₅=H, R₆=NO₂, R₇=OH, R₈=CH₃ R₃=4′-amidosulfonylphenylamidocarbonyl, R₄=R₅=H, R₆=NO₂, R₇=OH, R₈=CH₃; R₃=4′-guanidinosulfonylphenylamidocarbonyl, R₄=R₅=H, R₆=NO₂, R₇=OH, R₈=CH₃; R₃=4′-(2″-pyrimidinylamidosulfonyl)phenylamidocarbonyl, R₄=R₅=H, R₆=NO₂, R₇=OH, R₈=CH₃; R₃=4′-(5″,6″-dimethoxypyrimidinyl-4″-amidosulfonyl) phenylamidocarbonyl, R₄=R₅=H, R₆=NO₂, R₇=OH, R₈=CH₃; R₃=4′-(2″-thiazolamidosulfonyl)phenylamidocarbonyl, R₄=R₅=H, R₆=NO₂, R₇=OH, R₈=CH₃; R₃=o-CO₂H-p-I-phenylamidocarbonyl, R₄=R₅=H, R₆=NO₂, R₇=OH, R₈=CH₃; R₃=p-CO₂H-phenylamidocarbonyl, R₄=R₅=H, R₆=NO₂, R₇=OCH₃, R₈=CH₃; R₃=m-CO₂H-phenylamidocarbonyl, R₄=R₅=H, R_(6=NO) ₂, R₇=OCH₃, R₈=CH₃; R₃=o-CO₂H-phenylamidocarbonyl, R₄=R₅=H, R₆=NO₂, R₇=OCH₃, R₈=CH₃; R₃=p-OH-phenylamidocarbonyl, R₄=R₅=H, R₆=NO₂, R₇=OCH₃, R₈=CH₃; R₃=m-OH-phenylamidocarbonyl, R₄=R₅=H, R₆=NO₂, R₇=OCH₃, R₈=CH₃; R₃=o-OH-phenylamidocarbonyl, R₄=R₅=H, R₆=NO₂, R₇=OCH₃, R₈=CH₃; R₃=p-OCH₃-phenylamidocarbonyl, R₄=R₅=H, R₆=NO₂, R₇=OCH₃, R₈=CH₃; R₃=p-ethoxycarbophenylamidocarbonyl, R₄=R₅=H, R₆=NO₂, R₇=OCH₃, R₈=CH₃; R₃=m-OH-p-CO₂H-phenylamidocarbonyl, R₄=R₅=H, R₆=NO₂, R₇=OCH₃, R₈=CH₃; R₃=m-CF₃-phenylamidocarbonyl, R₄=R₅=H, R₆=NO₂, R₇=OCH₃, R₈=CH₃; R₃=m-CF₃-p-NO₂-phenylamidocarbonyl, R₄=R₅=H, R₆=NO₂, R₇=OCH₃, R₈=CH₃; R₃=4′-guanidinosulfonylphenylamidocarbonyl, R₄=R₅=H, R₆=NO₂, R₇=OCH₃, R₈=CH₃; R₃=4′-amidosufonylphenylamidocarbonyl, R₄=R₅=H, R₆=NO₂, R₇=OCH₃, R₈=CH₃; R₃=4′-(5″,6″-dimethoxypyrimidinyl-4″-amidosulfonyl) phenylamidocarbonyl, R₄=R₅=H, R₆=NO₂, R₇=OCH₃, R₈=CH₃; R₃=4′-(2″-thiazolamidosulfonyl)phenylamidocarbonyl, R₄=R₅=H, R₆=NO₂, R₇=OCH₃, R₈=CH₃; R₃=4′-(2″-pyrimidinylamidosulfonyl)phenylamidocarbonyl, R₄=R₅=H, R₆=NO₂, R₇=OCH₃, R₈=CH₃; R₃=p-CO₂H-phenylamidocarbonyl, R₄=R₅=H, R₆=R₈=NO₂, R₇=OH; R₃=p-OH-phenylamidocarbonyl, R₄=R₅=H, R₆=R₈=NO₂, R₇=OH; R₃=m-OH-phenylamidocarbonyl, R₄=R₅=H, R₆=R₈=NO₂, R₇=OH; R₃=o-OH-phenylamidocarbonyl, R₄=R₅=H, R₆=R₈=NO₂, R₇=OH; R₃=p-OCH₃-phenylamidocarbonyl, R₄=R₅=H, R₆=R₈=NO₂, R₇=OH; R₃=p-ethoxycarbophenylamidocarbonyl, R₄=R₅=H, R₆=R₈=NO₂, R₇=OH; R₃=CF₃-phenylamidocarbonyl, R₄=R₅=H, R₆=R₈=NO₂, R₇=OH; R₃=4′-amidosulfonylphenylamidocarbonyl, R₄=R₅=H, R₆=R₈=NO₂, R₇=OH; R₃=4′-guanidinosulfonylphenylamidocarbonyl, R₄=R₅=H, R₆=R₈=NO₂, R₇=OH; R₃=4′-(2″-pyrimidinylamidosulfonyl)phenylamidocarbonyl, R₄=R₅=H, R₆=R₈=NO₂, R₇=OH; R₃=4′-(5″,6″-dimethoxypyrimidinyl-4″-amidosulfonyl) phenylamidocarbonyl, R₄=R₅=H, R₆=R₈=NO₂, R₇=OH; R₃=4′-(2″-thiazolamidosulfonyl)phenylamidocarbonyl, R₄=R₅=H, R₆=R₈=NO₂, R₇=OH; R₃=o-CO₂H-phenylamidocarbonyl, R₄=R₅=H, R₆=R₈=NO₂, R₇=OH; R₃=p-OH-phenylamidocarbonyl, R₄=R₅=H, R₆=R₈=NO₂, R₇=OCH₃; R₃=p-ethoxycarbophenylamidocarbonyl, R₄=R₅=H, R₆=R₈=NO₂, R₇=OCH₃; R₃=p-OCH₃-phenylamidocarbonyl, R₄=R₅=H, R₆=R₈=NO₂, R₇=OCH₃; R₃=p-OCH₃-phenylamidocarbonyl, R₄=R₅=H, R₆=Cl, R₇=OH, R₈=NO₂; R₃=4′-guanidinosulfonylphenylamidocarbonyl, R₄=R₅=H, R₆=Cl, R₇=OH, R₈=NO₂; R₃=m-OH-pCO₂H-phenylamidocarbonyl, R₄=H, R₅=CH₃, R₇=OH, R₆=Cl, R₈=NO₂; R₃=p-CO₂H-phenylamidocarbonyl, R₄=H, R₅=CH₃, R₇=OH, R₆=R₈=NO₂; R₃=m-CO₂H-phenylamidocarbonyl, R₄=H, R₅=CH₃, R₇=OH, R₆=R₈=NO₂; R₃=o-CO₂H-phenylamidocarbonyl, R₄=H, R₅=CH₃, R₇=OH, R₆=R₈=NO₂; R₃=p-OCH₃-phenylamidocarbonyl, R₄=H, R₅=CH₃, R₇=OH, R₆=R₈=NO₂; R₃=p-ethoxycarbophenylamidocarbonyl, R₄=H, R₅=CH₃, R₇=OH, R₆=R₈=NO₂; R₃=p-amidosulfonylphenylamidocarbonyl, R₄=H, R₅=CH₃, R₇=OH, R₆=R₈=NO₂; R₃=p-guanidinosulfonylphenylamidocarbonyl, R₄=H, R₅=CH₃, R₇=OH, R₆=R₈=NO₂; R₃=4′-(2″-pyrimidinylamidosulfonyl)phenylamidocarbonyl, R₄=H, R₅=CH₃, R₇=OH, R₆=R₈=NO₂; R₃=4′-(2″-thiazolamidosulfonyl)phenylamidocarbonyl, R₄=H, R₅=CH₃, R₇=OH, R₆=R₈=NO₂; R₃=4′-(4″,6″-dimethylpyrimidinyl-2″-amidosulfonyl) phenylamidocarbonyl, R₄=H, R₅=CH₃, R₇=OH, R₆=R₈=NO₂;

R₃=CO₂C₂H₅, R₄=R₅=H, R₆=NO₂, R₇=R₈=OCH₃; R₃=CO₂H, R₄=R₅=H, R₆=NO₂, R₇=R₈=OCH₃; R₃=CO₂C₂H₅, R₄=R₅=H, R₆=NO₂, R₇=OH, R₈=CH₃; R₃=CO₂H, R₄=R₅=H, R₆=NO₂, R₇=OH, R₈=CH₃; R₃=CO₂C₂H₅, R₄=R₅=H, R₆=NH₂, R₇=OH, R₈=CH₃; R₃=CO₂H, R₄=R₅=H, R₆=NO₂, R₇=OCH₃, R₈=CH₃; R₃=CO₂C₂H₅, R₄=R₅=H, R₆=C₂H₅, R₇=OH, R₈=NO₂; R₃=CO₂H, R₄=R₅=H, R₆=C₂H₅, R₇=OH, R₈=NO₂; R₃=CO₂C₂H₅, R₄=R₅=H, R₆=C₂H₅, R₇=OCH₃, R₈=NO₂; R₃=CO₂H, R₄=R₅=H, R₆=C₂H₅, R₇=OCH₃, R₈=NO₂; R₃=CO₂C₂H₅, R₄=R₅=H, R₆=R₈=NO₂, R₇=OH; R₃=CO₂H, R₄=R₅=H, R₆=R₈=NO₂, R₇=OH; R₃=CO₂C₂H₅, R₄=R₅=H, R₆=R₈=NO₂, R₇=OCH₃; R₃=CO₂H, R₄=R₅=H, R₆=R₈=NO₂, R₇=OCH₃; R₃=CO₂C₂H₅, R₄=R₅=H, R₆=Cl, R₇=OH, R₈=NO₂; R₃=CO₂H, R₄=R₅=H, R₆=Cl, R₇=OH, R₈=NO₂; R₃=CO₂H, R₄=H, R₅=CH₃, R₆=R₈=NO₂, R₇=OH; R₃=CO₂C₂H₅, R₄=H, R₅=CH₃, R₆=R₈=NO₂, R₇=OH;


6. The compound according to claim 1, characterized in that the compound include the pharmaceutically acceptable salts and their hydrates, esters, or pro-drugs thereof.
 7. A method for the preparation of the compounds according to claim 1, characterized in condensing the substituted 3-carboxy-, 4-carboxy-, 6-carboxy-coumarin, or 7-carboxy-methylenoxy-coumarin derivative with a corresponding substituted amine or hydrazine.
 8. The method according to claim 7, characterized in condensing the substituted 3-carboxy-, 4-carboxy-, 6-carboxy-coumarin, or 7-carboxy-methylenoxy-coumarin derivative with corresponding substituted hydrazine, followed by cyclization of the so-obtained hydrazide to form the heterocyclic derivatives.
 9. The method according to claim 7, characterized in that reactants for the amidation reaction include phosphorus trichloride, phosphorus oxychloride, phosphorus pentachloride, thionyl chloride, 1,3-dicyclohexylcarbodiimide, dipyridylcarbonate (2-DPC), 1,3-diisopropylcarbodiimide (DIPC), and 1-(3-dimethylamino-propyl)-3-ethylcarbodiimide (EDCI); the catalytic agents used are selected from tert-amines, pyridine, 4-dimethylaminopyridine and pyrrolalkylpyridine; the organic solvents used comprising dimethylsulfoxide, dichloromethane, toluene, ethylene glycol dimethyl ether, 1,2-dichloroethane, tetrahydrofuran and N, N-dimethylformamide.
 10. A pharmaceutical composition characterized in comprising a pharmaceutically effective dosage of a compound according to claim 1, and a pharmaceutically acceptable carrier.
 11. The pharmaceutical composition according to claim 10, characterized in that, said the pharmaceutical composition is tablets, capsules, pills, injections, sustained-release, controlled-release or targeted preparations and various fine particle delivery systems.
 12. Use of a compound according to claim 1, for the preparation of inhibitors transforming growth factor β1 (TGF-β1).
 13. Use of a compound according to claim 1, for the preparation of antagonists of angiotensin II (AngII) receptor converting enzyme.
 14. Use of a compound according to claim 1, for the preparation of drugs for the treatment of chronic renal disorders.
 15. Use of a compound according to claim 1, for the preparation of drugs for the treatment of cardio-cerebrovascular diseases.
 16. Use of a compound according to claim 1, for the preparation of drugs for the treatment of non-insulin dependent diabetes.
 17. Use according to claim 15, characterized in that, said cardio-cerebrovascular diseases are hypertension, cerebral and coronary embolism, myocardial infarction, cerebrovascular accidents, stroke and their sequelae.
 18. Use of a compound according to claim 1, for the preparation of drugs for the treatment or prophylaxis of tumor and pre-cancerous lesions. 